Numb positively regulates autophagic flux via regulating lysosomal function
文献类型：期刊论文
| 作者 | Changan Jiang; Xianming Liu; Zhihao Ding; Ke Liu; Ximing Shao; Lei Zhang; Yi Liu; Haiyan Sun; Hongchang Li; Huashun Li |
| 刊名 | Biochemical and Biophysical Research Communications
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Autophagy is a lysosome-dependent catabolic process involving in the degradation and recycling of unnecessary or damaged proteins and organelles. Emerging evidence indicates that autophagy dysfunction is closely related to various human diseases including cancer, aging, myopathies and neurodegenerative disorders. Here, using genetic knockdown, we uncover the role of Numb, an endocytic adaptor protein, in regulating the late steps of autophagy. We found that Numb depletion led to the accumulation of autophagic vacuole, as verified by RFP-LC3 staining combined with transmission electron microscopy. Further investigation indicated that Numb depletion impaired autophagic degradation through inhibiting the activities of lysosomal enzymes (Cathepsin D, b-glucuronidase and b-glucosidase). Moreover, Numb depletion induced elevation of lysosomal pH values and decrease of glycosylated lysosome-associated membrane proteins.We further observed that Rab7 activity was inhibited in Numbdepleted cells. Together, our findings revealed a novel function of Numb and its likely mechanism in regulation of autophagy events. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12267]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | Biochemical and Biophysical Research Communications |
| 推荐引用方式 GB/T 7714 | Changan Jiang,Xianming Liu,Zhihao Ding,et al. Numb positively regulates autophagic flux via regulating lysosomal function[J]. Biochemical and Biophysical Research Communications,2017. |
| APA | Changan Jiang.,Xianming Liu.,Zhihao Ding.,Ke Liu.,Ximing Shao.,...&Huashun Li.(2017).Numb positively regulates autophagic flux via regulating lysosomal function.Biochemical and Biophysical Research Communications. |
| MLA | Changan Jiang,et al."Numb positively regulates autophagic flux via regulating lysosomal function".Biochemical and Biophysical Research Communications (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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