Multimodal nonlinear optical microscopy reveals critical role of kinesin-1 in cartilage development.
文献类型：期刊论文
| 作者 | Gan, Huiyan; Li, Xuesong; Sun, Qiqi; Duan, Zhigang; Xue, Wenqian; He, Sicong; Qu, Jianan Y; Huang, Jiandong |
| 刊名 | BIOMEDICAL OPTICS EXPRESS
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | We developed a multimodal nonlinear optical (NLO) microscope system by integrating stimulated Raman scattering (SRS), second harmonic generation (SHG) and twophoton excited fluorescence (TPEF) imaging. The system was used to study the morphological and biochemical characteristics of tibial cartilage in a kinesin-1 (Kif5b) knockout mouse model. The detailed structure of fibrillar collagen in the extracellular matrix of cartilage was visualized by the forward and backward SHG signals, while high resolution imaging of chondrocytes was achieved by capturing endogenous TPEF and SRS signals of the cells. The results demonstrate that collagen fibrils in the superficial surface of the articular cartilage decreased significantly in the absence of Kif5b. The distorted morphology along with accumulated intracellular collagen was observed in the Kif5b-deficient chondrocytes, indicating the critical roles of kinesin-1 in the chondrocyte morphogenesis and collagen secretion. The study shows that multimodal NLO imaging method is an effective approach to investigate early development of cartilage. (C) 2017 Optical Society of America |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12325]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | BIOMEDICAL OPTICS EXPRESS |
| 推荐引用方式 GB/T 7714 | Gan, Huiyan,Li, Xuesong,Sun, Qiqi,et al. Multimodal nonlinear optical microscopy reveals critical role of kinesin-1 in cartilage development.[J]. BIOMEDICAL OPTICS EXPRESS,2017. |
| APA | Gan, Huiyan.,Li, Xuesong.,Sun, Qiqi.,Duan, Zhigang.,Xue, Wenqian.,...&Huang, Jiandong.(2017).Multimodal nonlinear optical microscopy reveals critical role of kinesin-1 in cartilage development..BIOMEDICAL OPTICS EXPRESS. |
| MLA | Gan, Huiyan,et al."Multimodal nonlinear optical microscopy reveals critical role of kinesin-1 in cartilage development.".BIOMEDICAL OPTICS EXPRESS (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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