Dissecting nucleosome function with a comprehensive histone H2A and H2B mutant library
文献类型：期刊论文
| 作者 | Shen, Yue; Jiang, Shuangying; Liu, Yan; Xu, Caiyue; Wang, Yun; Gong, Jianhui; Wu, Qingyu; Boeke, Jef D; Dai, Junbiao |
| 刊名 | G3-Genes Genomes Genetics
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Using a comprehensive library of histone H2A and H2B mutants, we assessed the biological function of each amino acid residue involved in various stress conditions including exposure to different DNA damage-inducing reagents, different growth temperatures and other chemicals. H2B N- and H2A C-termini were critical for maintaining nucleosome function and mutations in these regions led to pleiotropic phenotypes. Additionally, two screens were performed using this library, monitoring heterochromatin gene silencing and genome stability, to identify residues which could compromise normal function when mutated. Many distinctive regions within the nucleosome were revealed. Furthermore, we used the bar-seq method to profile the mutant composition of many libraries in one high-throughput sequencing experiment, greatly reducing the labor and increasing the capacity. This study not only demonstrates the applications of the versatile histone library, but also revealed many previously unknown functions of histone H2A and H2B. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12334]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | G3-Genes Genomes Genetics |
| 推荐引用方式 GB/T 7714 | Shen, Yue,Jiang, Shuangying,Liu, Yan,et al. Dissecting nucleosome function with a comprehensive histone H2A and H2B mutant library[J]. G3-Genes Genomes Genetics,2017. |
| APA | Shen, Yue.,Jiang, Shuangying.,Liu, Yan.,Xu, Caiyue.,Wang, Yun.,...&Dai, Junbiao.(2017).Dissecting nucleosome function with a comprehensive histone H2A and H2B mutant library.G3-Genes Genomes Genetics. |
| MLA | Shen, Yue,et al."Dissecting nucleosome function with a comprehensive histone H2A and H2B mutant library".G3-Genes Genomes Genetics (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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