Response of live Newcastle disease virus encapsulated in N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan nanoparticles.
文献类型：期刊论文
| 作者 | Wang, Xiaohua; Jin, Zheng; Li, Dan; Dai, Chunxiao; Cheng, Guogang; Zhao, Kai |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | We have synthesized N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan (N-2-HFCC) with higher water solubility than chitosan. To evaluate its potential as delivery carrier, NDV encapsulated in N-2-HFCC/CMC nanoparticles (NDV/La Sota-N-2-HFCC/CMC-NPs) with a diameter of 252.2 +/- 32.68 nm, Zeta potential of 41.1 +/- 0.89 mV, encapsulation efficiency of 96.6 +/- 0.72% and loading capacity of 53.2 +/- 1.11% were prepared. NDV/La Sota-N-2-HFCC/CMC-NPs are a novel type of attenuated live vaccine encapsulated in nanoparticles. These nanoparticles displayed lower cytotoxicity and higher stability. Their bioactivity was maintained when they were stored for three months at the room temperature. Release assay in vitro showed that NDV could be sustainably released after an initial burst release. In vivo immunization showed that immunization of specific pathogen free chickens with NDV/La Sota-N-2-HFCC/CMC-NPs intranasally induced high titers of serum antibody, significantly promoted lymphocyte proliferation and caused higher levels of interleukine-2 and interference-gamma. These results indicated that N-2-HFCC/CMC nanoparticles could serve as an efficient and safe delivery vehicle for mucosal immunity, and have a great potential in medical applications. (C) 2017 Elsevier Ltd. All rights reserved. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12341]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | CARBOHYDRATE POLYMERS |
| 推荐引用方式 GB/T 7714 | Wang, Xiaohua,Jin, Zheng,Li, Dan,et al. Response of live Newcastle disease virus encapsulated in N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan nanoparticles.[J]. CARBOHYDRATE POLYMERS,2017. |
| APA | Wang, Xiaohua,Jin, Zheng,Li, Dan,Dai, Chunxiao,Cheng, Guogang,&Zhao, Kai.(2017).Response of live Newcastle disease virus encapsulated in N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan nanoparticles..CARBOHYDRATE POLYMERS. |
| MLA | Wang, Xiaohua,et al."Response of live Newcastle disease virus encapsulated in N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan nanoparticles.".CARBOHYDRATE POLYMERS (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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