A noninvasive online system for biomass monitoring in shaker flasks using backward scattered light
文献类型：期刊论文
| 作者 | Jian Mao; Zuowei Wang; Xianshuai Chen; Yingge Yan; Olaf Eichstädt; Jinming Cui |
| 刊名 | Biotechnology and Bioprocess Engineering
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | This paper presents a noninvasive optical sensor system for monitoring cell growth in shaker flasks commonly used in biological laboratories. The system uses an open-source microprocessor board to monitor concentration of Escherichia coli host cells. To allow measurement for a range of filling degrees and shaker speeds, the backscattering angle is chosen to minimize interference from surface reflections and the measurement window is synchronized to the position of the shaker flask. A nonlinear calibration model of scattered light can predict offline optical density with a mean relative error of 5.2%, an accuracy which is comparable to the classical offline method and sufficient for biotechnology applications. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/11513]  |
| 专题 | 深圳先进技术研究院_南沙所 |
| 作者单位 | Biotechnology and Bioprocess Engineering |
| 推荐引用方式 GB/T 7714 | Jian Mao,Zuowei Wang,Xianshuai Chen,et al. A noninvasive online system for biomass monitoring in shaker flasks using backward scattered light[J]. Biotechnology and Bioprocess Engineering,2017. |
| APA | Jian Mao,Zuowei Wang,Xianshuai Chen,Yingge Yan,Olaf Eichstädt,&Jinming Cui.(2017).A noninvasive online system for biomass monitoring in shaker flasks using backward scattered light.Biotechnology and Bioprocess Engineering. |
| MLA | Jian Mao,et al."A noninvasive online system for biomass monitoring in shaker flasks using backward scattered light".Biotechnology and Bioprocess Engineering (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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