miR-4632 mediates PDGF-BB-induced proliferation and antiapoptosis of human pulmonary artery smooth muscle cells via targeting cJUN
文献类型：期刊论文
| 作者 | Gou, Deming; Li, Xiang ; Chen, Jidong ; Li, Yanjiao ; Qian, Zhengjiang |
| 刊名 | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | MicroRNAs (miRNAs) can regulate the proliferative status of pulmonary artery smooth muscle cells (PASMCs), which is a core factor modulating pulmonary vascular remodeling diseases, such as atherosclerosis and pulmonary arterial hypertension (PAH). Our previous work has shown that miR-4632, a rarely reported miRNA, is significantly downregulated in platelet-derived growth factor (PDGF)-BB-stimulated human pulmonary artery smooth muscle cells (HPASMCs), yet its cell function and the underlying molecular mechanisms remain to be elucidated. Here, we find that miR-4632 is highly expressed in HPASMCs and its expression significantly decreased in response to different stimuli. Functional studies revealed that miR-4632 inhibited proliferation and promoted apoptosis of HPASMCs but had no effects on cell contraction and migration. Furthermore, the cJUN was identified as a direct target gene of miR-4632, while knockdown of cJUN was necessary for miR-4632-mediated HPASMC proliferation and apoptosis. In addition, the downregulation of miR-4632 by PDGF-BB was found to associate with histone deacetylation through the activation of PDGF receptor/phosphatidylinositol 3′-kinase/histone deacetylase 4 signaling. Finally, the expression of miR-4632 was reduced in the serum of patients with PAH. Overall, our results suggest that miR-4632 plays an important role in regulating HPASMC proliferation and apoptosis by suppression of cJUN, providing a novel therapeutic miRNA candidate for the treatment of pulmonary vascular remodeling diseases. It also implies that serum miR-4632 has the potential to serve as a circulating biomarker for PAH diagnosis. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/11914]  |
| 专题 | 深圳先进技术研究院_脑所 |
| 作者单位 | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY |
| 推荐引用方式 GB/T 7714 | Gou, Deming, Li, Xiang , Chen, Jidong ,et al. miR-4632 mediates PDGF-BB-induced proliferation and antiapoptosis of human pulmonary artery smooth muscle cells via targeting cJUN[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2017. |
| APA | Gou, Deming, Li, Xiang , Chen, Jidong , Li, Yanjiao ,&Qian, Zhengjiang .(2017).miR-4632 mediates PDGF-BB-induced proliferation and antiapoptosis of human pulmonary artery smooth muscle cells via targeting cJUN.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY. |
| MLA | Gou, Deming,et al."miR-4632 mediates PDGF-BB-induced proliferation and antiapoptosis of human pulmonary artery smooth muscle cells via targeting cJUN".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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