Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Liu, X; Zhou, Y; Chen, HB; Peng, SL; Gu, YC; Ding, LS; Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China. |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2008 |
| 卷号 | 22期号:12页码:1981_1992 |
| ISSN号 | 0951-4198 |
| 产权排序 | 1 |
| 英文摘要 | A rapid, simple electrospray ionization mass spectrometry (ESI-MS) sensitivity enhancement method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine hydrochloride (NH2OH center dot HCl) as a derivatization reagent. We use the oxime formed during the derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups. Copyright (C) 2008 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000256985100025 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18079]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Liu, X,Zhou, Y,Chen, HB,et al. Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2008,22(12):1981_1992. |
| APA | Liu, X.,Zhou, Y.,Chen, HB.,Peng, SL.,Gu, YC.,...&Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China..(2008).Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,22(12),1981_1992. |
| MLA | Liu, X,et al."Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22.12(2008):1981_1992. |
入库方式： OAI收割
来源：成都生物研究所
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