A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, using ISSR markers
文献类型:期刊论文
作者 | Hao, BQ; Li, W; Mu, LC; Li, Y; Rui, Z; Tang, MX; Bao, WK; Li, W, Sichuan Univ, Sch Life Sci, Chengdu 610064, Peoples R China. |
刊名 | BIOCHEMICAL GENETICS
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出版日期 | 2006 |
卷号 | 44期号:1_2页码:31_45 |
关键词 | Cupressus Chengiana Issr Genetic Diversity Genetic Structure Differentiation |
ISSN号 | 0006-2928 |
产权排序 | 1 |
英文摘要 | ISSR markers were used to analyze the genetic diversity and genetic structure of eight natural populations of Cupressus chengiana in China. ISSR analysis using 10 primers was carried out on 92 different samples. At the species level, 136 polymorphic loci were detected. The percentage of polymorphic bands (PPB) was 99%. Genetic diversity (H (e)) was 0.3120, effective number of alleles (A (e)) was 1.5236, and Shannon's information index (I) was 0.4740. At the population level, PPB = 48%, A (e)=1.2774, H (e)=0.1631, and I=0.2452. Genetic differentiation (G (st)) detected by Nei's genetic diversity analysis suggested 48% occurred among populations. The partitioning of molecular variance by AMOVA analysis indicated significant genetic differentiation within populations (54%) and among populations (46%; P < 0.0003). The average number of individuals exchanged between populations per generation (N (m) ) was 0.5436. Samples from the same population clustered in the same population-specific cluster, and two groups of Sichuan and Gansu populations were distinguishable. A significantly positive correlation between genetic and geographic distance was detected (r=0.6701). Human impacts were considered one of the main factors to cause the rarity of C. chengiana, and conservation strategies are suggested based on the genetic characters and field investigation, e.g., protection of wild populations, reestablishment of germplasm bank, and reintroduction of more genetic diversity. |
学科主题 | Biochemistry & Molecular Biology ; Genetics & Heredity |
语种 | 英语 |
公开日期 | 2011-07-08 |
源URL | [http://210.75.237.14/handle/351003/21886] ![]() |
专题 | 成都生物研究所_生态研究 |
通讯作者 | Li, W, Sichuan Univ, Sch Life Sci, Chengdu 610064, Peoples R China. |
推荐引用方式 GB/T 7714 | Hao, BQ,Li, W,Mu, LC,et al. A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, using ISSR markers[J]. BIOCHEMICAL GENETICS,2006,44(1_2):31_45. |
APA | Hao, BQ.,Li, W.,Mu, LC.,Li, Y.,Rui, Z.,...&Li, W, Sichuan Univ, Sch Life Sci, Chengdu 610064, Peoples R China..(2006).A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, using ISSR markers.BIOCHEMICAL GENETICS,44(1_2),31_45. |
MLA | Hao, BQ,et al."A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, using ISSR markers".BIOCHEMICAL GENETICS 44.1_2(2006):31_45. |
入库方式: OAI收割
来源:成都生物研究所
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