De novo assembly and characterization of leaf transcriptome for the development of EST-SSR markers of the non-model species Indigofera szechuensis
文献类型：期刊论文
| 作者 | Guo, LN; Zhao, XL; Gao, XF; Gao, XF (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu 610041, Sichuan, Peoples R China.; Gao, XF (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ecol Restorat Biodivers Conservat Key Lab Sichuan, Chengdu 610041, Sichuan, Peoples R China. |
| 刊名 | BIOCHEMICAL SYSTEMATICS AND ECOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 68页码:36-43 |
| 关键词 | Indigofera Szechuensis Transcriptome Functional Annotation Ssr Sites |
| DOI | 10.1016/j.bse.2016.06.010 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Indigofera szechuensis Craib, a species endemic to Hengduan Mountains in China, is widely distributed across a range of habitats, including slopes, trailsides, and riverbanks. This species serves as a good model to examine flexible adaptation of plants. In this study, we reported the transcriptome sequence of this species and developed a set of SSR markers for such evolutionary studies. Approximately 19.4 million clean reads from the leaves of I. szechuensis were generated through transcriptome sequencing, and 43,896 unigenes with an average length of 798.54 bp were obtained. A total of 27,320 (62.2%) unigenes were annotated for their functions, and 17,422 and 8017 unigenes could be aligned to the GO and COG database, respectively. By searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG), 5682 unigenes were assigned to 118 KEGG pathways that belong to five main categories, i.e., organismal systems, cellular processes, environmental information processing, metabolism, and genetic information processing. We further identified 4052 potential SSR sites. Using 102 randomly selected EST-SSRs, 73 markers were successfully amplified with the expected size in L szechuensis and 67-71 markers of these 73 SSR5 in eight other Indigofera species. We firstly established a public trascriptome platform for the species-rich genus Indigofera. The SSR sites identified here provide additional resources for development of molecular markers for further evolutionary studies for this species and congeners. Published by Elsevier Ltd. |
| 学科主题 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| 资助项目 | 中国科学院山地生态恢复与生物资源利用重点实验室 |
| 语种 | 英语 |
| 资助机构 | National Natural Science Foundation of China [31370230, 31070173] ; National Natural Science Foundation of China [31370230, 31070173] ; National Natural Science Foundation of China [31370230, 31070173] ; National Natural Science Foundation of China [31370230, 31070173] |
| 源URL | [http://210.75.237.14/handle/351003/28103]  |
| 专题 | 成都生物研究所_生态研究 |
| 通讯作者 | Gao, XF (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu 610041, Sichuan, Peoples R China.; Gao, XF (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ecol Restorat Biodivers Conservat Key Lab Sichuan, Chengdu 610041, Sichuan, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Guo, LN,Zhao, XL,Gao, XF,et al. De novo assembly and characterization of leaf transcriptome for the development of EST-SSR markers of the non-model species Indigofera szechuensis[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2016,68:36-43. |
| APA | Guo, LN,Zhao, XL,Gao, XF,Gao, XF ,&Gao, XF .(2016).De novo assembly and characterization of leaf transcriptome for the development of EST-SSR markers of the non-model species Indigofera szechuensis.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,68,36-43. |
| MLA | Guo, LN,et al."De novo assembly and characterization of leaf transcriptome for the development of EST-SSR markers of the non-model species Indigofera szechuensis".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 68(2016):36-43. |
入库方式： OAI收割
来源：成都生物研究所
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