Molecular cloning and functional characterization of a cinnamate 4-hydroxylase-encoding gene from Camptotheca acuminata
文献类型：期刊论文
| 作者 | Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China.; Li, W; Yang, LX; Jiang, LZ; Zhang, GL; Luo, YG; Luo, YG (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China. |
| 刊名 | ACTA PHYSIOLOGIAE PLANTARUM
 |
| 出版日期 | 2016 |
| 卷号 | 38期号:11页码:256（1-9） |
| 关键词 | Cinnamate 4-hydroxylase Camptotheca Acuminata P450 P-coumaric Acid Phenylpropanoid |
| DOI | 10.1007/s11738-016-2275-7 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Cinnamate 4-hydroxylase (C4H) catalyzes the regioselective para-hydroxylation of trans-cinnamic acid to form p-coumaric acid, the biosynthetic precursor of phenylpropanoid-based polymers. These biopolymers play an essential role in plant structure construction, development, and defense. Herein the open reading frame of CaC4H2 was cloned from Camptotheca acuminata, a deciduous camptothecin-producing tree native to China. CaC4H2 showed 94 % amino acid residues identity with those of reported CaC4H, which suggested that CaC4H2 is an isoform of C4Hs presented in C. acuminata. The intact CaC4H2 was overexpressed in Escherichia coli with its functional reaction partner cytochrome P450 reductase, CamCPR, which transfers electrons from NADPH to CaC4H2 to support the catalytic hydroxylation activity of CaC4H2. Upon incubating trans-cinnamic acid with the recombinant CaC4H2 and tCamCPR, the formation of pcoumaric acid was confirmed by the HPLC-DAD and UPLC-DAD-ESIMS analyses, which indicated the catalytic hydroxylation activity of CaC4H2. Quantitative realtime PCR analyses showed that CaC4H2 was expressed in all tissues of C. acuminata seedlings, which is consistent with the well-known conclusion that the C4H-catalyzed hydroxylation reaction is a key step within the biosynthetic pathway of phenylpropanoids. The functional characterization of CaC4H2 will be useful for molecular breeding and sustainable utilization and protection of the camptothecin-producing plant. |
| 学科主题 | Plant Sciences |
| 语种 | 英语 |
| 资助机构 | Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; National Natural Science Foundation of China [21172216] ; National Natural Science Foundation of China [21172216] ; Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; National Natural Science Foundation of China [21172216] ; National Natural Science Foundation of China [21172216] |
| 源URL | [http://210.75.237.14/handle/351003/28172]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China.; Luo, YG (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Luo, YG ,Li, W,Yang, LX,et al. Molecular cloning and functional characterization of a cinnamate 4-hydroxylase-encoding gene from Camptotheca acuminata[J]. ACTA PHYSIOLOGIAE PLANTARUM,2016,38(11):256（1-9）. |
| APA | Luo, YG .,Li, W.,Yang, LX.,Jiang, LZ.,Zhang, GL.,...&Luo, YG .(2016).Molecular cloning and functional characterization of a cinnamate 4-hydroxylase-encoding gene from Camptotheca acuminata.ACTA PHYSIOLOGIAE PLANTARUM,38(11),256（1-9）. |
| MLA | Luo, YG ,et al."Molecular cloning and functional characterization of a cinnamate 4-hydroxylase-encoding gene from Camptotheca acuminata".ACTA PHYSIOLOGIAE PLANTARUM 38.11(2016):256（1-9）. |
入库方式： OAI收割
来源：成都生物研究所
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