Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli
文献类型：期刊论文
| 作者 | Luo, YG; Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China.; Luo, YG (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China.; Li, W; Chen, F; Pu, X; Yang, Y; Jiang, LZ; Zhang, GL |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 43期号:9页码:1281-1292 |
| 关键词 | Geraniol Synthase Camptotheca Acuminata Geraniol Camptothecin Metabolic Engineering Fermentation |
| DOI | 10.1007/s10295-016-1802-2 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Geraniol synthase (GES) catalyzes the conversion of geranyl diphosphate (GPP) into geraniol, an acyclic monoterpene alcohol that has been widely used in many industries. Here we report the functional characterization of CaGES from Camptotheca acuminata, a camptothecin-producing plant, and its application in production of geraniol in Escherichia coli. The full-length cDNA of CaGES was obtained from overlap extension PCR amplification. The intact and N-terminus-truncated CaGESs were overexpressed in E. coli and purified to homogeneity. Recombinant CaGES showed the conversion activity from GPP to geraniol. To produce geraniol in E. coli using tCaGES, the biosynthetic precursor GPP should be supplied and transferred to the catalytic pocket of tCaGES. Thus, ispA(S80F), a mutant of farnesyl diphosphate (FPP) synthase, was prepared to produce GPP via the head-to-tail condensation of isoprenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). A slight increase of geraniol production was observed in the fermentation broth of the recombinant E. coli harboring tCaGES and ispA(S80F). To enhance the supply of IPP and DMAPP, the encoding genes involved in the whole mevalonic acid biosynthetic pathway were introduced to the E. coli harboring tCaGES and the ispA(S80F) and a significant increase of geraniol yield was observed. The geraniol production was enhanced to 5.85 +/- 0.46 mg L-1 when another copy of ispA(S80F) was introduced to the above recombinant strain. The following optimization of medium composition, fermentation time, and addition of metal ions led to the geraniol production of 48.5 +/- 0.9 mg L-1. The present study will be helpful to uncover the biosynthetic enigma of camptothecin and tCaGES will be an alternative to selectively produce geraniol in E. coli with other metabolic engineering approaches. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 资助机构 | Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; National Natural Science Foundation of China [21172216] ; National Natural Science Foundation of China [21172216] ; Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Science and Technology Project for Outstanding Youths in Life Science from the Chinese Academy of Sciences [KSCX2-EW-Q-6] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; National Natural Science Foundation of China [21172216] ; National Natural Science Foundation of China [21172216] |
| 源URL | [http://210.75.237.14/handle/351003/28180]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China.; Luo, YG (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Luo, YG,Luo, YG ,Luo, YG ,et al. Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(9):1281-1292. |
| APA | Luo, YG.,Luo, YG .,Luo, YG .,Li, W.,Chen, F.,...&Zhang, GL.(2016).Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(9),1281-1292. |
| MLA | Luo, YG,et al."Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.9(2016):1281-1292. |
入库方式： OAI收割
来源：成都生物研究所
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