Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism
文献类型：期刊论文
| 作者 | Liu, YM (reprint author), Jackson State Univ, Dept Chem & Biochem, 1400 Lynch St, Jackson, MS 39217 USA.; Wan, LH; Jiang, XL; Liu, YM; Hu, JJ; Liang, J; Liao, X; Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China. |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 408期号:9页码:2275-2283 |
| 关键词 | Lipase Inhibitors Scutellaria Baicalensis Magnetic Solid Phase Extraction Fluorescence Spectroscopy Molecular Docking |
| DOI | 10.1007/s00216-016-9320-7 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC50 values were determined to be 229.22 +/- 12.67, 153.71 +/- 9.21, and 56.07 +/- 4.90 mu M, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 资助机构 | National Natural Science Foundation of China [81173536] ; National Natural Science Foundation of China [81173536] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; U.S. National Institutes of Health [GM 089557] ; U.S. National Institutes of Health [GM 089557] ; National Natural Science Foundation of China [81173536] ; National Natural Science Foundation of China [81173536] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; U.S. National Institutes of Health [GM 089557] ; U.S. National Institutes of Health [GM 089557] |
| 源URL | [http://210.75.237.14/handle/351003/28205]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Liu, YM (reprint author), Jackson State Univ, Dept Chem & Biochem, 1400 Lynch St, Jackson, MS 39217 USA.; Liu, YM; Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Liu, YM ,Wan, LH,Jiang, XL,et al. Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(9):2275-2283. |
| APA | Liu, YM .,Wan, LH.,Jiang, XL.,Liu, YM.,Hu, JJ.,...&Liao, X .(2016).Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(9),2275-2283. |
| MLA | Liu, YM ,et al."Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.9(2016):2275-2283. |
入库方式： OAI收割
来源：成都生物研究所
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