Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection
文献类型：期刊论文
| 作者 | Wang, MQ (reprint author), Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China.; Tang, Z; Zhang, HF; Dong, J; Wang, MQ; Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 刊名 | ORGANIC & BIOMOLECULAR CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 14期号:7页码:2347-2351 |
| DOI | 10.1039/c6ob00148c |
| 产权排序 | 2 |
| 文献子类 | Article |
| 英文摘要 | We identified a new class of deoxyribozymes named A-2 and A-3 by in vitro selection which required both Cu2+ and Mn2+ as cofactors that selectively and rapidly cleave the DNA substrate. Studies confirmed that they cleaved via a mechanistic pathway involving the formation of hydrogen peroxide as the reactive species. The kinetics, secondary structures and sequence tolerance of the new class of the deoxyribozymes A-2 and A-3 were reported. |
| 学科主题 | Chemistry |
| 语种 | 英语 |
| 资助机构 | Senior Talent Cultivation Program of Jiangsu University [15JDG068] ; Senior Talent Cultivation Program of Jiangsu University [15JDG068] ; National Natural Science Foundation of China [21402189, 21322208] ; National Natural Science Foundation of China [21402189, 21322208] ; China Postdoctoral Science Foundation [2014M560733] ; China Postdoctoral Science Foundation [2014M560733] ; Senior Talent Cultivation Program of Jiangsu University [15JDG068] ; Senior Talent Cultivation Program of Jiangsu University [15JDG068] ; National Natural Science Foundation of China [21402189, 21322208] ; National Natural Science Foundation of China [21402189, 21322208] ; China Postdoctoral Science Foundation [2014M560733] ; China Postdoctoral Science Foundation [2014M560733] |
| 源URL | [http://210.75.237.14/handle/351003/28220]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Wang, MQ (reprint author), Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China.; Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Wang, MQ ,Tang, Z,Zhang, HF,et al. Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2016,14(7):2347-2351. |
| APA | Wang, MQ ,Tang, Z,Zhang, HF,Dong, J,Wang, MQ,&Tang, Z .(2016).Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection.ORGANIC & BIOMOLECULAR CHEMISTRY,14(7),2347-2351. |
| MLA | Wang, MQ ,et al."Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection".ORGANIC & BIOMOLECULAR CHEMISTRY 14.7(2016):2347-2351. |
入库方式： OAI收割
来源：成都生物研究所
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