ITS non-concerted evolution and rampant hybridization in the legume genus Lespedeza (Fabaceae)
文献类型：期刊论文
| 作者 | Xu, B; Zeng, XM; Gao, XF; Jin, DP; Zhang, LB; Gao, XF (reprint author), Chinese Acad Sci, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Chengdu Inst Biol, POB 416, Chengdu 610041, Sichuan, Peoples R China.; Gao, XF (reprint author), Chinese Acad Sci, Ecol Restorat & Biodivers Conservat Key Lab Sichu, Chengdu Inst Biol, POB 416, Chengdu 610041, Sichuan, Peoples R China.; Zhang, LB (reprint author), Missouri Bot Garden, POB 299, St Louis, MO 63166 USA. |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7页码:1-15 |
| DOI | 10.1038/srep40057 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | The internal transcribed spacer (ITS) as one part of nuclear ribosomal DNA is one of the most extensively sequenced molecular markers in plant systematics. The ITS repeats generally exhibit high-level within-individual homogeneity, while relatively small-scale polymorphism of ITS copies within individuals has often been reported in literature. Here, we identified large-scale polymorphism of ITS copies within individuals in the legume genus Lespedeza (Fabaceae). Divergent paralogs of ITS sequences, including putative pseudogenes, recombinants, and multiple functional ITS copies were sometimes detected in the same individual. Thirty-seven ITS pseudogenes could be easily detected according to nucleotide changes in conserved 5.8S motives, the significantly lower GC contents in at least one of three regions, and the lost ability of 5.8S rDNA sequence to fold into a conserved secondary structure. The distribution patterns of the putative functional clones were highly different between the traditionally recognized two subgenera, suggesting different rates of concerted evolution in two subgenera which could be attributable to their different extents/frequencies of hybridization, confirmed by our analysis of the single-copy nuclear gene PGK. These findings have significant implications in using ITS marker for reconstructing phylogeny and studying hybridization. |
| 学科主题 | Science & Technology - Other Topics |
| 项目编号 | 中国科学院山地生态恢复与生物资源利用重点实验室 |
| 语种 | 英语 |
| 资助机构 | We are grateful to Xue-Li Zhao, Zhang-Ming Zhu, Meng Li, and Li-Na Guo for their help with fieldwork. Special thanks go to Heng-Ning Deng for his assistance in the lab. This research was supported by the National Natural Science Foundation of China (Grant No. 31200169, 31570196). the China Postdoctoral Science Foundation (Grant No. 2012M511946), Project of Platform Construction for Plant Resources of Sichuan Province (2016TJPT0001-3), and a grant from the Fondation Franklinia to the Flora of China project. ; National Natural Science Foundation of China [31200169, 31570196] ; We are grateful to Xue-Li Zhao, Zhang-Ming Zhu, Meng Li, and Li-Na Guo for their help with fieldwork. Special thanks go to Heng-Ning Deng for his assistance in the lab. This research was supported by the National Natural Science Foundation of China (Grant No. 31200169, 31570196). the China Postdoctoral Science Foundation (Grant No. 2012M511946), Project of Platform Construction for Plant Resources of Sichuan Province (2016TJPT0001-3), and a grant from the Fondation Franklinia to the Flora of China project. ; National Natural Science Foundation of China [31200169, 31570196] ; China Postdoctoral Science Foundation [2012M511946] ; China Postdoctoral Science Foundation [2012M511946] ; Project of Platform Construction for Plant Resources of Sichuan Province [2016TJPT0001-3] ; Project of Platform Construction for Plant Resources of Sichuan Province [2016TJPT0001-3] ; Fondation Franklinia ; Fondation Franklinia ; We are grateful to Xue-Li Zhao, Zhang-Ming Zhu, Meng Li, and Li-Na Guo for their help with fieldwork. Special thanks go to Heng-Ning Deng for his assistance in the lab. This research was supported by the National Natural Science Foundation of China (Grant No. 31200169, 31570196). the China Postdoctoral Science Foundation (Grant No. 2012M511946), Project of Platform Construction for Plant Resources of Sichuan Province (2016TJPT0001-3), and a grant from the Fondation Franklinia to the Flora of China project. ; National Natural Science Foundation of China [31200169, 31570196] ; We are grateful to Xue-Li Zhao, Zhang-Ming Zhu, Meng Li, and Li-Na Guo for their help with fieldwork. Special thanks go to Heng-Ning Deng for his assistance in the lab. This research was supported by the National Natural Science Foundation of China (Grant No. 31200169, 31570196). the China Postdoctoral Science Foundation (Grant No. 2012M511946), Project of Platform Construction for Plant Resources of Sichuan Province (2016TJPT0001-3), and a grant from the Fondation Franklinia to the Flora of China project. ; National Natural Science Foundation of China [31200169, 31570196] ; China Postdoctoral Science Foundation [2012M511946] ; China Postdoctoral Science Foundation [2012M511946] ; Project of Platform Construction for Plant Resources of Sichuan Province [2016TJPT0001-3] ; Project of Platform Construction for Plant Resources of Sichuan Province [2016TJPT0001-3] ; Fondation Franklinia ; Fondation Franklinia |
| 源URL | [http://210.75.237.14/handle/351003/29161]  |
| 专题 | 成都生物研究所_生态研究 |
| 通讯作者 | Gao, XF (reprint author), Chinese Acad Sci, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Chengdu Inst Biol, POB 416, Chengdu 610041, Sichuan, Peoples R China.; Gao, XF (reprint author), Chinese Acad Sci, Ecol Restorat & Biodivers Conservat Key Lab Sichu, Chengdu Inst Biol, POB 416, Chengdu 610041, Sichuan, Peoples R China.; Zhang, LB (reprint author), Missouri Bot Garden, POB 299, St Louis, MO 63166 USA. |
| 推荐引用方式 GB/T 7714 | Xu, B,Zeng, XM,Gao, XF,et al. ITS non-concerted evolution and rampant hybridization in the legume genus Lespedeza (Fabaceae)[J]. SCIENTIFIC REPORTS,2017,7:1-15. |
| APA | Xu, B.,Zeng, XM.,Gao, XF.,Jin, DP.,Zhang, LB.,...&Zhang, LB .(2017).ITS non-concerted evolution and rampant hybridization in the legume genus Lespedeza (Fabaceae).SCIENTIFIC REPORTS,7,1-15. |
| MLA | Xu, B,et al."ITS non-concerted evolution and rampant hybridization in the legume genus Lespedeza (Fabaceae)".SCIENTIFIC REPORTS 7(2017):1-15. |
入库方式： OAI收割
来源：成都生物研究所
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