Loss of Tmem30a leads to photoreceptor degeneration
文献类型：期刊论文
| 作者 | Zhang, L; Yang, YM; Li, SJ; Zhang, SS; Zhu, X; Tai, ZF; Yang, M; Liu, YQ; Guo, XZ; Chen, B |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7页码:9296(1-14) |
| DOI | 10.1038/s41598-017-09506-5 |
| 产权排序 | 4 |
| 文献子类 | Article |
| 英文摘要 | Phosphatidylserine (PS) is asymmetrically distributed between the outer and inner leaflets of the plasma membrane in eukaryotic cells. PS asymmetry on the plasma membrane depends on the activities of P4-ATPases, and disruption of PS distribution can lead to various disease conditions. Folding and transporting of P4-ATPases to their cellular destination requires the beta subunit TMEM30A proteins. However, the in vivo functions of Tmem30a remain unknown. To this end, we generated retinal-specific Tmem30a-knockout mice to investigate its roles in vivo for the first time. Our data demonstrated that loss of Tmem30a in mouse cone cells leads to mislocalization of cone opsin, loss of photopic electroretinogram (ERG) responses and loss of cone cells. Mechanistically, Tmem30a-mutant mouse embryonic fibroblasts (MEFs) exhibited diminished PS flippase activity and increased exposure of PS on the cell surface. The broad loss of Tmem30a in adult mice led to a reduced scotopic photoresponse, mislocalization of ATP8A2 to the inner segment and cell body, and increased apoptosis in the retina. Our data demonstrated novel essential roles of Tmem30a in the retina. |
| 学科主题 | Science & Technology - Other Topics |
| 项目编号 | 天然药物和临床转化实验室 |
| 语种 | 英语 |
| 资助机构 | The authors want to thank the patients for their participation. The authors also want to thank Dr. Robert Molday for generously providing TMEM30A, ATP8A2, and 1D1, 1D4 antibodies, Dr. Simon SW John for providing the vmd mouse line and Dr. Y.Z. Le for providing the HRGP-Cre (cone-Cre) mouse line. This study was supported by grants from the Natural Science Foundation of China (81470668, 81271007, [XJ.Zhu.], 81670877 [F.Lu]), the Department of Science and Technology of Sichuan Province (2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122 [XJ.Zhu]), and the Sichuan Provincial Outstanding Youth Research Grant (2014JQ0023 [XJ.Zhu]), grant from China Postdoctoral Science Foundation (2016M600734) to Lin Zhang. The authors alone are responsible for the content and the writing of the paper. ; Natural Science Foundation of China [81470668, 81271007, 81670877] ; The authors want to thank the patients for their participation. The authors also want to thank Dr. Robert Molday for generously providing TMEM30A, ATP8A2, and 1D1, 1D4 antibodies, Dr. Simon SW John for providing the vmd mouse line and Dr. Y.Z. Le for providing the HRGP-Cre (cone-Cre) mouse line. This study was supported by grants from the Natural Science Foundation of China (81470668, 81271007, [XJ.Zhu.], 81670877 [F.Lu]), the Department of Science and Technology of Sichuan Province (2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122 [XJ.Zhu]), and the Sichuan Provincial Outstanding Youth Research Grant (2014JQ0023 [XJ.Zhu]), grant from China Postdoctoral Science Foundation (2016M600734) to Lin Zhang. The authors alone are responsible for the content and the writing of the paper. ; Natural Science Foundation of China [81470668, 81271007, 81670877] ; Department of Science and Technology of Sichuan Province [2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122] ; Department of Science and Technology of Sichuan Province [2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122] ; Sichuan Provincial Outstanding Youth Research Grant [2014JQ0023] ; Sichuan Provincial Outstanding Youth Research Grant [2014JQ0023] ; China Postdoctoral Science Foundation [2016M600734] ; China Postdoctoral Science Foundation [2016M600734] ; The authors want to thank the patients for their participation. The authors also want to thank Dr. Robert Molday for generously providing TMEM30A, ATP8A2, and 1D1, 1D4 antibodies, Dr. Simon SW John for providing the vmd mouse line and Dr. Y.Z. Le for providing the HRGP-Cre (cone-Cre) mouse line. This study was supported by grants from the Natural Science Foundation of China (81470668, 81271007, [XJ.Zhu.], 81670877 [F.Lu]), the Department of Science and Technology of Sichuan Province (2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122 [XJ.Zhu]), and the Sichuan Provincial Outstanding Youth Research Grant (2014JQ0023 [XJ.Zhu]), grant from China Postdoctoral Science Foundation (2016M600734) to Lin Zhang. The authors alone are responsible for the content and the writing of the paper. ; Natural Science Foundation of China [81470668, 81271007, 81670877] ; The authors want to thank the patients for their participation. The authors also want to thank Dr. Robert Molday for generously providing TMEM30A, ATP8A2, and 1D1, 1D4 antibodies, Dr. Simon SW John for providing the vmd mouse line and Dr. Y.Z. Le for providing the HRGP-Cre (cone-Cre) mouse line. This study was supported by grants from the Natural Science Foundation of China (81470668, 81271007, [XJ.Zhu.], 81670877 [F.Lu]), the Department of Science and Technology of Sichuan Province (2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122 [XJ.Zhu]), and the Sichuan Provincial Outstanding Youth Research Grant (2014JQ0023 [XJ.Zhu]), grant from China Postdoctoral Science Foundation (2016M600734) to Lin Zhang. The authors alone are responsible for the content and the writing of the paper. ; Natural Science Foundation of China [81470668, 81271007, 81670877] ; Department of Science and Technology of Sichuan Province [2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122] ; Department of Science and Technology of Sichuan Province [2016TD0009, 2017TJPT0010, 2015SC0060, 2014FZ0122] ; Sichuan Provincial Outstanding Youth Research Grant [2014JQ0023] ; Sichuan Provincial Outstanding Youth Research Grant [2014JQ0023] ; China Postdoctoral Science Foundation [2016M600734] ; China Postdoctoral Science Foundation [2016M600734] |
| 源URL | [http://210.75.237.14/handle/351003/29215]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Lu, F; Zhu, XJ (reprint author), Hosp Univ Elect Sci & Technol China, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China.; Zhu, XJ (reprint author), Hosp Univ Elect Sci & Technol China, Sch Med, Chengdu 610072, Sichuan, Peoples R China.; Zhu, XJ (reprint author), Sichuan Prov Peoples Hosp, Chengdu 610072, Sichuan, Peoples R China.; Zhu, XJ (reprint author), Sichuan Acad Med Sci, Dept Lab Med, Chengdu 610072, Sichuan, Peoples R China.; Zhu, XJ (reprint author), Sichuan Acad Med Sci, Inst Lab Anim Sci, Chengdu, Sichuan, Peoples R China.; Zhu, XJ (reprint author), Chinese Acad Sci, Sichuan Translat Med Res Hosp, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Zhang, L,Yang, YM,Li, SJ,et al. Loss of Tmem30a leads to photoreceptor degeneration[J]. SCIENTIFIC REPORTS,2017,7:9296(1-14). |
| APA | Zhang, L.,Yang, YM.,Li, SJ.,Zhang, SS.,Zhu, X.,...&Zhu, XJ .(2017).Loss of Tmem30a leads to photoreceptor degeneration.SCIENTIFIC REPORTS,7,9296(1-14). |
| MLA | Zhang, L,et al."Loss of Tmem30a leads to photoreceptor degeneration".SCIENTIFIC REPORTS 7(2017):9296(1-14). |
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来源：成都生物研究所
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