Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKC beta and focal adhesion kinase
文献类型：期刊论文
| 作者 | Shaheen, S; Wan, ZP; Li, ZY; Chau, A; Li, XX; Zhang, SS; Liu, Y; Yi, JY; Zeng, YY; Wang, J |
| 刊名 | ELIFE
 |
| 出版日期 | 2017 |
| 卷号 | 6页码:1-29 |
| DOI | 10.7554/eLife.23060 |
| 产权排序 | 4 |
| 文献子类 | Article |
| 英文摘要 | The mechanosensing ability of lymphocytes regulates their activation in response to antigen stimulation, but the underlying mechanism remains unexplored. Here, we report that B cell mechanosensing-governed activation requires BCR signaling molecules. PMA-induced activation of PKC beta can bypass the Btk and PLC-gamma 2 signaling molecules that are usually required for B cells to discriminate substrate stiffness. Instead, PKC beta-dependent activation of FAK is required, leading to FAK-mediated potentiation of B cell spreading and adhesion responses. FAK inactivation or deficiency impaired B cell discrimination of substrate stiffness. Conversely, adhesion molecules greatly enhanced this capability of B cells. Lastly, B cells derived from rheumatoid arthritis (RA) patients exhibited an altered BCR response to substrate stiffness in comparison with healthy controls. These results provide a molecular explanation of how initiation of B cell activation discriminates substrate stiffness through a PKC beta-mediated FAK activation dependent manner. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| 资助机构 | Ministry of Science and Technology of the People's Republic of China 2014CB542500 Wanli Liu; National Science Foundation 81422020 Wanli Liu; National Science Foundation 81621002 Wanli Liu ; Ministry of Science and Technology of the People's Republic of China [2014CB542500] ; Ministry of Science and Technology of the People's Republic of China 2014CB542500 Wanli Liu; National Science Foundation 81422020 Wanli Liu; National Science Foundation 81621002 Wanli Liu ; Ministry of Science and Technology of the People's Republic of China [2014CB542500] ; National Science Foundation [81422020, 81621002] ; National Science Foundation [81422020, 81621002] ; Ministry of Science and Technology of the People's Republic of China 2014CB542500 Wanli Liu; National Science Foundation 81422020 Wanli Liu; National Science Foundation 81621002 Wanli Liu ; Ministry of Science and Technology of the People's Republic of China [2014CB542500] ; Ministry of Science and Technology of the People's Republic of China 2014CB542500 Wanli Liu; National Science Foundation 81422020 Wanli Liu; National Science Foundation 81621002 Wanli Liu ; Ministry of Science and Technology of the People's Republic of China [2014CB542500] ; National Science Foundation [81422020, 81621002] ; National Science Foundation [81422020, 81621002] |
| 源URL | [http://210.75.237.14/handle/351003/29222]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Liu, WL (reprint author), Tsinghua Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Key Lab Prot Sci, Sch Life Sci,Inst Immunol,MOE, Beijing, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Shaheen, S,Wan, ZP,Li, ZY,et al. Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKC beta and focal adhesion kinase[J]. ELIFE,2017,6:1-29. |
| APA | Shaheen, S.,Wan, ZP.,Li, ZY.,Chau, A.,Li, XX.,...&Liu, WL .(2017).Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKC beta and focal adhesion kinase.ELIFE,6,1-29. |
| MLA | Shaheen, S,et al."Substrate stiffness governs the initiation of B cell activation by the concerted signaling of PKC beta and focal adhesion kinase".ELIFE 6(2017):1-29. |
入库方式： OAI收割
来源：成都生物研究所
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