An efficient direct competitive nano-ELISA for residual BSA determination in vaccines
文献类型：期刊论文
| 作者 | Qing, LS; Luo, P (reprint author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macau, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Key Lab Nat Med & Translat Med, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China.; Tao, WJ; Wang, QL; Li, J; Li, XD; Ding, LS; Luo, P |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 409期号:19页码:4607-4614 |
| 关键词 | Bovine Serum Albumin Vaccines Sio2 Nanoparticles Direct Competitive Nano-elisa |
| DOI | 10.1007/s00216-017-0403-x |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | A simple, fast, and highly sensitive direct competitive enzyme-linked immunosorbent assay (ELISA) based on bovine serum albumin (BSA) antigen labeled amine-terminated silicon dioxide (SiO2-NH-BSA) nanoparticles was developed to determine residual BSA in vaccines. As nano-ELISA using nanomaterials with a very high surface-to-volume ratio has emerged as a promising strategy, SiO2-NH-BSA nanoparticles were prepared in this study by the coupling of BSA to SiO2 nanoparticles modified with amidogen, followed by the quantification of BSA via a direct competitive binding of BSA-antigen-labeled SiO2 nanoparticles to anti-BSA antibody conjugated with horseradish peroxidase. The validation study showed that the linear range of this method was from 1 to 90 ng/mL (r = 0.998) and the limit of detection was 0.67 ng/mL. The intra-assay and interassay coefficients of variation were less than 10% at three concentrations (10, 40, and 70 ng/mL), and the recovery was 92.4%, indicating good specificity. As a proof of principle, this new method was applied in the analysis of residual BSA in five different vaccines. Bland-Altman plots revealed that there was no significant difference in the accuracy and precision between our new method and the most commonly used sandwich ELISA. From the results taken together, the new method developed in this study is more sensitive and facile with lower cost and thus demonstrated potential to be applied in the quality control of biological products. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| 项目编号 | 中国科学院山地生态恢复与生物资源利用重点实验室 ; 天然药物和临床转化实验室 |
| 语种 | 英语 |
| 资助机构 | Financial support was provided by the National Natural Science Foundation of China (no. 21202161) and the Macao Science and Technology Development Fund (no. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Financial support was provided by the National Natural Science Foundation of China (no. 21202161) and the Macao Science and Technology Development Fund (no. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Macao Science and Technology Development Fund [052/2013/A2] ; Macao Science and Technology Development Fund [052/2013/A2] ; Financial support was provided by the National Natural Science Foundation of China (no. 21202161) and the Macao Science and Technology Development Fund (no. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Financial support was provided by the National Natural Science Foundation of China (no. 21202161) and the Macao Science and Technology Development Fund (no. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Macao Science and Technology Development Fund [052/2013/A2] ; Macao Science and Technology Development Fund [052/2013/A2] |
| 源URL | [http://210.75.237.14/handle/351003/29229]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Luo, P (reprint author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macau, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Key Lab Nat Med & Translat Med, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Qing, LS,Luo, P ,Qing, LS ,et al. An efficient direct competitive nano-ELISA for residual BSA determination in vaccines[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(19):4607-4614. |
| APA | Qing, LS.,Luo, P .,Qing, LS .,Tao, WJ.,Wang, QL.,...&Luo, P.(2017).An efficient direct competitive nano-ELISA for residual BSA determination in vaccines.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(19),4607-4614. |
| MLA | Qing, LS,et al."An efficient direct competitive nano-ELISA for residual BSA determination in vaccines".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.19(2017):4607-4614. |
入库方式： OAI收割
来源：成都生物研究所
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