中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 成都生物研究所
  3. 中国科学院成都生物研究所
  4. 天然产物研究
Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine

文献类型：期刊论文

作者Xie, J; Li, XD; Ding, LS; Liang, J; Luo, P; Qing, LS; Luo, P (reprint author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Nat Med & Translat Med, Chengdu 610041, Peoples R China.; Wang, QL
刊名TALANTA
出版日期2017
卷号167期号:2017页码:617-622
关键词Enzyme-linked Ligand Binding Assay Nano-sio2 Ibuprofen Direct Competitive Hsa
DOI10.1016/j.talanta.2017.03.011
产权排序1
文献子类Article
英文摘要The application domains of classic enzyme-linked ligand binding assay (ELBA) is relatively narrow due to the high cost and hardly available binding receptor. In here, we described for the first time the possibility of developing a new ELBA based on silica nanoparticles (nano-SiO2) to assess the ibuprofen in human urine. Nano-SiO2 with a large surface area was introduced as stationary phase to improve the analytical performance. In the experiment, a competitively binding procedure with human serum albumin (HSA) was performed between the ibuprofen presented in sample and horseradish peroxidase labeled ibuprofen (HRP-ibuprofen) subsequently added. After centrifugal separation, the HRP/ibuprofen/nano-SiO2 composite catalyzed the substrate solution (TMB/H2O2) with a color change from colorless to yellow for quantitative measurement via an ultraviolet spectrophotometer. As a validation of the new principle, the developed nano-ELBA method was applied in the determination of ibuprofen excreted in human urine with excellent performance. This detection range only depends on the solubility of ligand and sensitivity of UV spectrophotometer. Our results indicate that this new method demonstrated to be able to rapidly and adequately determine the concentration of components in biological samples and advocate its effectiveness for various applications.
学科主题Chemistry
项目编号中国科学院山地生态恢复与生物资源利用重点实验室
语种英语
资助机构This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Macao Science and Technology Development Fund [052/2013/A2] ; Macao Science and Technology Development Fund [052/2013/A2] ; This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Macao Science and Technology Development Fund [052/2013/A2] ; Macao Science and Technology Development Fund [052/2013/A2]
源URL[http://210.75.237.14/handle/351003/29237]  
专题成都生物研究所_天然产物研究
通讯作者Luo, P (reprint author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Nat Med & Translat Med, Chengdu 610041, Peoples R China.
推荐引用方式
GB/T 7714		 Xie, J,Li, XD,Ding, LS,et al. Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine[J]. TALANTA,2017,167(2017):617-622.
APA Xie, J.,Li, XD.,Ding, LS.,Liang, J.,Luo, P.,...&Wang, QL.(2017).Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine.TALANTA,167(2017),617-622.
MLA Xie, J,et al."Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine".TALANTA 167.2017(2017):617-622.

入库方式： OAI收割

来源：成都生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。