Enhanced production of camptothecin and biological preparation of N-1-acetylkynuramine in Camptotheca acuminata cell suspension cultures
文献类型：期刊论文
| 作者 | Luo, YG (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China.; Luo, YG; Yang, Y; Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China.; Pu, X; Qu, XX; Chen, F; Zhang, GL |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 101期号:10页码:4053-4062 |
| 关键词 | Camptotheca Acuminata Camptothecin Cell Suspension Cultures Sorbitol N-1-acetylkynuramine |
| DOI | 10.1007/s00253-017-8153-4 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | The Camptotheca acuminata cell suspension cultures were established to produce the well-known antitumor monoterpene indole alkaloid camptothecin (CAM). Most CAM was present in the broth of the C. acuminata cell suspension cultures. The CAM production was evidenced to be attenuated when the C. acuminata cell suspension cultures were continuously subcultured and grown under identical axenic conditions. A practical cryopreservation and recovery procedure was established to maintain the C. acuminata cell suspension cultures. Biotic and abiotic elicitors were administrated to the C. acuminata cell suspension cultures to restore and enhance CAM production. Of them, sorbitol, a well-known hyperosmotic stressor, was proven to be the most effective elicitor that stimulates a similar to 500-fold increase of CAM production. The committed biosynthetic precursors of CAM, tryptamine and secologanin, were feed to the C. acuminata cell suspension cultures and the CAM production is not remarkably increased. However, N (1)-acetylkynuramine (NAK), an important metabolite of kynuramine pathway, was isolated and identified from the cell suspension cultures feeding with tryptamine. The present work provides an efficient method to produce CAM and NAK using the C. acuminata cell suspension cultures. The biotransformation of tryptamine to NAK sheds lights on the biosynthetic formation of the pyrroloquinoline moiety of CAM. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 资助机构 | This work was supported in part by the KSCX2-EW-Q-6 and ZHTS-003 projects from the Chinese Academy of Sciences, the Applied and Basic Research Program of Sichuan Province (2015JY0058), and the 21172216 project from the National Natural Science Foundation of China. ; Chinese Academy of Sciences [KSCX2-EW-Q-6, ZHTS-003] ; This work was supported in part by the KSCX2-EW-Q-6 and ZHTS-003 projects from the Chinese Academy of Sciences, the Applied and Basic Research Program of Sichuan Province (2015JY0058), and the 21172216 project from the National Natural Science Foundation of China. ; Chinese Academy of Sciences [KSCX2-EW-Q-6, ZHTS-003] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; National Natural Science Foundation of China [21172216] ; National Natural Science Foundation of China [21172216] ; This work was supported in part by the KSCX2-EW-Q-6 and ZHTS-003 projects from the Chinese Academy of Sciences, the Applied and Basic Research Program of Sichuan Province (2015JY0058), and the 21172216 project from the National Natural Science Foundation of China. ; Chinese Academy of Sciences [KSCX2-EW-Q-6, ZHTS-003] ; This work was supported in part by the KSCX2-EW-Q-6 and ZHTS-003 projects from the Chinese Academy of Sciences, the Applied and Basic Research Program of Sichuan Province (2015JY0058), and the 21172216 project from the National Natural Science Foundation of China. ; Chinese Academy of Sciences [KSCX2-EW-Q-6, ZHTS-003] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; Applied and Basic Research Program of Sichuan Province [2015JY0058] ; National Natural Science Foundation of China [21172216] ; National Natural Science Foundation of China [21172216] |
| 源URL | [http://210.75.237.14/handle/351003/29241]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Luo, YG (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China.; Luo, YG (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ctr Nat Prod Res, Chengdu 610041, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Luo, YG ,Luo, YG,Yang, Y,et al. Enhanced production of camptothecin and biological preparation of N-1-acetylkynuramine in Camptotheca acuminata cell suspension cultures[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(10):4053-4062. |
| APA | Luo, YG .,Luo, YG.,Yang, Y.,Luo, YG .,Pu, X.,...&Zhang, GL.(2017).Enhanced production of camptothecin and biological preparation of N-1-acetylkynuramine in Camptotheca acuminata cell suspension cultures.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(10),4053-4062. |
| MLA | Luo, YG ,et al."Enhanced production of camptothecin and biological preparation of N-1-acetylkynuramine in Camptotheca acuminata cell suspension cultures".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.10(2017):4053-4062. |
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来源：成都生物研究所
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