Characterization of major metabolites of polymethoxylated flavonoids in Pericarpium Citri Reticulatae using liver microsomes immobilized on magnetic nanoparticles coupled with UPLC/MS-MS
文献类型：期刊论文
| 作者 | Liu, YM; Lei, J; Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China.; Liao, X; Xue, Y |
| 刊名 | CHEMISTRY CENTRAL JOURNAL
 |
| 出版日期 | 2017 |
| 卷号 | 11页码:13（1-10） |
| 关键词 | Liver Microsome Magnetic Nanoparticles Metabolism Polymethoxylated Flavonoids Pericarpium Citri Reticulatae |
| DOI | 10.1186/s13065-017-0237-9 |
| 产权排序 | 3 |
| 文献子类 | Article |
| 英文摘要 | The peels of citrus fruits (Pericarpium Citri Reticulatae, PCR) have long been used in traditional Chinese medicines (TCMs). Polymethoxylated flavonoids (PMFs) were found to be the main components present in PCR extracts, but their metabolism remains unclear which restrain the utilization of this TCM. In the present work, rat liver microsomes were immobilized on magnetic nanoparticles (LMMNPs) for in vitro metabolic study on the whole PMFs of PCR. LMMNPs were characterized by transmission electron microscope and Fourier-transform infrared spectrum. The relative enzyme binding capacity of LMMNPs was estimated to be about 428 mu g/mg from thermogravimetric analysis. Incubation of LMMNPs with PMFs produced demethylated metabolites of PMFs, six of which were identified by ultra-high pressure liquid chromatography-mass spectrometry (UPLC-MS/MS). The 3'-hydroxylated tangeretin (T3) was detected from the metabolites of tangeretin for the first time, which suggested that 4'-demethylated and 3'-hydroxylated derivative of tangeretin (3'-hydroxy-5,6,7,8,4'-pentamethoxyflavone, T4) was not only derived from 4'-demethylated tangeretin (T2) as previously reported, but also from T3. This is the first investigation of the metabolism of the whole PMFs, which may shed light on the mechanism of action of PCR. |
| 学科主题 | Chemistry |
| 语种 | 英语 |
| 资助机构 | Financial support from the National Science Foundation of China (No. 81173536), the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12), and the West Light Foundation of the Chinese Academy of Sciences is gratefully acknowledged. ; National Science Foundation of China [81173536] ; Financial support from the National Science Foundation of China (No. 81173536), the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12), and the West Light Foundation of the Chinese Academy of Sciences is gratefully acknowledged. ; National Science Foundation of China [81173536] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; West Light Foundation of the Chinese Academy of Sciences ; West Light Foundation of the Chinese Academy of Sciences ; Financial support from the National Science Foundation of China (No. 81173536), the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12), and the West Light Foundation of the Chinese Academy of Sciences is gratefully acknowledged. ; National Science Foundation of China [81173536] ; Financial support from the National Science Foundation of China (No. 81173536), the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12), and the West Light Foundation of the Chinese Academy of Sciences is gratefully acknowledged. ; National Science Foundation of China [81173536] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; West Light Foundation of the Chinese Academy of Sciences ; West Light Foundation of the Chinese Academy of Sciences |
| 源URL | [http://210.75.237.14/handle/351003/29253]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Liu, YM,Lei, J,Liao, X ,et al. Characterization of major metabolites of polymethoxylated flavonoids in Pericarpium Citri Reticulatae using liver microsomes immobilized on magnetic nanoparticles coupled with UPLC/MS-MS[J]. CHEMISTRY CENTRAL JOURNAL,2017,11:13（1-10）. |
| APA | Liu, YM,Lei, J,Liao, X ,Liao, X,&Xue, Y.(2017).Characterization of major metabolites of polymethoxylated flavonoids in Pericarpium Citri Reticulatae using liver microsomes immobilized on magnetic nanoparticles coupled with UPLC/MS-MS.CHEMISTRY CENTRAL JOURNAL,11,13（1-10）. |
| MLA | Liu, YM,et al."Characterization of major metabolites of polymethoxylated flavonoids in Pericarpium Citri Reticulatae using liver microsomes immobilized on magnetic nanoparticles coupled with UPLC/MS-MS".CHEMISTRY CENTRAL JOURNAL 11(2017):13（1-10）. |
入库方式： OAI收割
来源：成都生物研究所
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