Screening of novel RGD peptides to modify nanoparticles for targeted cancer therapy
文献类型：期刊论文
| 作者 | Huang, KQ; Ge, L; Zhang, YA (reprint author), Third Mil Med Univ, Xinqiao Hosp, Dept Orthoped, Chongqing 100037, Peoples R China.; Wu, J (reprint author), Nanjing iPharma Technol Co Ltd, 68 Zhushap Rd, Nanjing 211100, Jiangsu, Peoples R China.; Wu, J (reprint author), Sun Yat Sen Univ, Sch Engn, Guangzhou 510006, Guangdong, Peoples R China.; Qian, H (reprint author), China Pharmaceut Univ, State Key Lab Nat Med, 24 Tongjia Xiang, Nanjing 210009, Jiangsu, Peoples R China.; Qian, H; Wu, J; Zhang, YA; Ren, Y |
| 刊名 | BIOMATERIALS SCIENCE
 |
| 出版日期 | 2017 |
| 卷号 | 6期号:1页码:125-135 |
| DOI | 10.1039/c7bm00776k |
| 产权排序 | 3 |
| 文献子类 | Article |
| 英文摘要 | New targeted RGD peptides obtained by solid phase peptide synthesis (SPPS) were successfully screened by Molecular Operating Environment (MOE) and used for the building of the 6-O-carboxymethyl chitosan based carrier with an active target on the surface. CRGDYC-6-O-carboxymethyl chitosan based nanoparticles (NPs) loaded with doxorubicin hydrochloride (DOX) were successfully prepared by an ionic gelation method with the carrier synthesized before. Synthesis conditions and formulation parameters were optimized by determining the characteristics of nanoparticles including the particle size and drug encapsulation efficiency. 6-O-Carboxymethyl chitosan concentration, calcium chloride concentration and calcium chloride/6-O-carboxymethyl chitosan ratio all had effects on the particle size and drug encapsulation efficiency. Nanoparticles with an average diameter of 193.4 nm, an average drug loading efficiency of up to 69.5% and an average drug loading of up to 0.395% were prepared successfully with the optimal formulation. Flow cytometry and confocal microscopy analyses showed that the cellular uptake of DOX in human breast cancer cell lines (MCF-7) was higher in the CRGDYC-modified nanoparticles compared with the unmodified nanoparticles. In vivo imaging showed that the distribution of CRGDYC-modified nanoparticles in the tumor site was higher compared with the unmodified nanoparticles. These results suggest that CRGDYC-6-O-carboxymethyl chitosan may be a promising cancer targeting carrier which can enhance the intracellular uptake and cytotoxicity of the drug-loaded nanoparticles. |
| 学科主题 | Materials Science |
| 项目编号 | 中国科学院山地生态恢复与生物资源利用重点实验室 |
| 语种 | 英语 |
| 资助机构 | This work was supported by the National Natural Science Foundation of China (no. 81572978 & 81673299 & 21704104), the Natural Science Foundation of Jiangsu Province (no. BK2012355) and the Top-notch Academic Programs Project of Jiangsu Higher Education, the Thousand Talents Plan for Young Professionals, Guangdong Innovative and Entrepreneurial Research Team Program (2013S086), the Guangdong Natural Science Foundation (2014A030312018), and the Science and Technology Planning Project of Guangdong Province (2016A010103015). Meanwhile, we also want to sincerely acknowledge the funding from the Science and Technology Program of Guangzhou (201707010094). ; National Natural Science Foundation of China [81572978, 81673299, 21704104] ; This work was supported by the National Natural Science Foundation of China (no. 81572978 & 81673299 & 21704104), the Natural Science Foundation of Jiangsu Province (no. BK2012355) and the Top-notch Academic Programs Project of Jiangsu Higher Education, the Thousand Talents Plan for Young Professionals, Guangdong Innovative and Entrepreneurial Research Team Program (2013S086), the Guangdong Natural Science Foundation (2014A030312018), and the Science and Technology Planning Project of Guangdong Province (2016A010103015). Meanwhile, we also want to sincerely acknowledge the funding from the Science and Technology Program of Guangzhou (201707010094). ; National Natural Science Foundation of China [81572978, 81673299, 21704104] ; Natural Science Foundation of Jiangsu Province [BK2012355] ; Natural Science Foundation of Jiangsu Province [BK2012355] ; Top-notch Academic Programs Project of Jiangsu Higher Education ; Top-notch Academic Programs Project of Jiangsu Higher Education ; Thousand Talents Plan for Young Professionals ; Thousand Talents Plan for Young Professionals ; Guangdong Innovative and Entrepreneurial Research Team Program [2013S086] ; Guangdong Innovative and Entrepreneurial Research Team Program [2013S086] ; Guangdong Natural Science Foundation [2014A030312018] ; Guangdong Natural Science Foundation [2014A030312018] ; Science and Technology Planning Project of Guangdong Province [2016A010103015] ; Science and Technology Planning Project of Guangdong Province [2016A010103015] ; Science and Technology Program of Guangzhou [201707010094] ; Science and Technology Program of Guangzhou [201707010094] ; This work was supported by the National Natural Science Foundation of China (no. 81572978 & 81673299 & 21704104), the Natural Science Foundation of Jiangsu Province (no. BK2012355) and the Top-notch Academic Programs Project of Jiangsu Higher Education, the Thousand Talents Plan for Young Professionals, Guangdong Innovative and Entrepreneurial Research Team Program (2013S086), the Guangdong Natural Science Foundation (2014A030312018), and the Science and Technology Planning Project of Guangdong Province (2016A010103015). Meanwhile, we also want to sincerely acknowledge the funding from the Science and Technology Program of Guangzhou (201707010094). ; National Natural Science Foundation of China [81572978, 81673299, 21704104] ; This work was supported by the National Natural Science Foundation of China (no. 81572978 & 81673299 & 21704104), the Natural Science Foundation of Jiangsu Province (no. BK2012355) and the Top-notch Academic Programs Project of Jiangsu Higher Education, the Thousand Talents Plan for Young Professionals, Guangdong Innovative and Entrepreneurial Research Team Program (2013S086), the Guangdong Natural Science Foundation (2014A030312018), and the Science and Technology Planning Project of Guangdong Province (2016A010103015). Meanwhile, we also want to sincerely acknowledge the funding from the Science and Technology Program of Guangzhou (201707010094). ; National Natural Science Foundation of China [81572978, 81673299, 21704104] ; Natural Science Foundation of Jiangsu Province [BK2012355] ; Natural Science Foundation of Jiangsu Province [BK2012355] ; Top-notch Academic Programs Project of Jiangsu Higher Education ; Top-notch Academic Programs Project of Jiangsu Higher Education ; Thousand Talents Plan for Young Professionals ; Thousand Talents Plan for Young Professionals ; Guangdong Innovative and Entrepreneurial Research Team Program [2013S086] ; Guangdong Innovative and Entrepreneurial Research Team Program [2013S086] ; Guangdong Natural Science Foundation [2014A030312018] ; Guangdong Natural Science Foundation [2014A030312018] ; Science and Technology Planning Project of Guangdong Province [2016A010103015] ; Science and Technology Planning Project of Guangdong Province [2016A010103015] ; Science and Technology Program of Guangzhou [201707010094] ; Science and Technology Program of Guangzhou [201707010094] |
| 源URL | [http://210.75.237.14/handle/351003/29257]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 通讯作者 | Zhang, YA (reprint author), Third Mil Med Univ, Xinqiao Hosp, Dept Orthoped, Chongqing 100037, Peoples R China.; Wu, J (reprint author), Nanjing iPharma Technol Co Ltd, 68 Zhushap Rd, Nanjing 211100, Jiangsu, Peoples R China.; Wu, J (reprint author), Sun Yat Sen Univ, Sch Engn, Guangzhou 510006, Guangdong, Peoples R China.; Qian, H (reprint author), China Pharmaceut Univ, State Key Lab Nat Med, 24 Tongjia Xiang, Nanjing 210009, Jiangsu, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Huang, KQ,Ge, L,Zhang, YA ,et al. Screening of novel RGD peptides to modify nanoparticles for targeted cancer therapy[J]. BIOMATERIALS SCIENCE,2017,6(1):125-135. |
| APA | Huang, KQ.,Ge, L.,Zhang, YA .,Wu, J .,Wu, J .,...&You, XR.(2017).Screening of novel RGD peptides to modify nanoparticles for targeted cancer therapy.BIOMATERIALS SCIENCE,6(1),125-135. |
| MLA | Huang, KQ,et al."Screening of novel RGD peptides to modify nanoparticles for targeted cancer therapy".BIOMATERIALS SCIENCE 6.1(2017):125-135. |
入库方式： OAI收割
来源：成都生物研究所
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