Mitochondria Targeted and Intracellular Biothiol Triggered Hyperpolarized Xe-129 Magnetofluorescent Biosensor
文献类型：期刊论文
| 作者 | Zhang, Bin2; Yang, Yuqi2; Zeng, Qingbin1,2; Guo, Qianni1,2; Zhou, Xin1,2; Bouchard, Louis-S.3,4; Liu, Maili1,2; Luo, Qing1,2; Yuan, Yaping1,2; Zhang, Xiaoxiao2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-02-21 |
| 卷号 | 89期号:4页码:2288-2295 |
| DOI | 10.1021/acs.analchem.6b03742 |
| 文献子类 | Article |
| 英文摘要 | Biothiols such as gluthathione (GSH), cysteine (Cys), homocysteine (Hcy), and thioredoxin (Trx) play vital roles in cellular metabolism. Various diseases are associated with abnormal cellular biothiol levels. Thus, the intracellular detection of biothiol levels could be a useful diagnostic tool. A number of methods have been developed to detect intracellular thiols, but sensitivity and specificity problems have limited their applications. To address these limitations, we have designed a new biosensor based on hyperpolarized xenon magnetic resonance detection, which can be used to detect biothiol levels noninvasively. The biosensor is a multimodal probe that incorporates a cryptophane-A cage as Xe-129 NMR reporter, a naphthalimide moiety as fluorescence reporter, a disulfide bond as thiol-specific cleavable group, and a triphenylphosphonium moiety as mitochondria targeting unit. When the biosensor interacts with biothiols, disulfide bond cleavage leads to enhancements in the fluorescence intensity and changes in the Xe-129 chemical shift. Using Hyper-CEST (chemical exchange saturation transfer) NMR, our biosensor shows a low detection limit at picomolar (10(-10) M) concentration, which makes a promise to detect thiols in cells. The biosensor can detect biothiol effectively in live cells and shows good targeting ability to the mitochondria. This new approach not only offers a practical technique to detect thiols in live cells, but may also present an excellent in vivo test platform for xenon biosensors. |
| WOS关键词 | 2-PHOTON FLUORESCENT-PROBE ; XENON NMR-SPECTROSCOPY ; MAMMALIAN-CELLS ; CANCER-CELLS ; 129XE MRI ; IN-VIVO ; GLUTATHIONE ; THIOLS ; SENSOR ; GAS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000394724700018 |
| 资助机构 | National Natural Science Foundation of China(81227902 ; National Natural Science Foundation of China(81227902 ; National Science Fund for Distinguished Young Scholars(81625011) ; National Science Fund for Distinguished Young Scholars(81625011) ; 21302217 ; 21302217 ; 21475147) ; 21475147) ; National Natural Science Foundation of China(81227902 ; National Natural Science Foundation of China(81227902 ; National Science Fund for Distinguished Young Scholars(81625011) ; National Science Fund for Distinguished Young Scholars(81625011) ; 21302217 ; 21302217 ; 21475147) ; 21475147) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/10873]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Collaborat Innovat Ctr Chem Life Sci,Wuhan Inst P, Natl Ctr Magnet Resonance Wuhan,Key Lab Magnet Re, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Calif Nano Syst Inst, Dept Chem & Biochem,Inst Mol Biol, Los Angeles, CA 90095 USA 4.Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Calif Nano Syst Inst, Dept Bioengn,Inst Mol Biol, Los Angeles, CA 90095 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Bin,Yang, Yuqi,Zeng, Qingbin,et al. Mitochondria Targeted and Intracellular Biothiol Triggered Hyperpolarized Xe-129 Magnetofluorescent Biosensor[J]. ANALYTICAL CHEMISTRY,2017,89(4):2288-2295. |
| APA | Zhang, Bin.,Yang, Yuqi.,Zeng, Qingbin.,Guo, Qianni.,Zhou, Xin.,...&Ren, Lili.(2017).Mitochondria Targeted and Intracellular Biothiol Triggered Hyperpolarized Xe-129 Magnetofluorescent Biosensor.ANALYTICAL CHEMISTRY,89(4),2288-2295. |
| MLA | Zhang, Bin,et al."Mitochondria Targeted and Intracellular Biothiol Triggered Hyperpolarized Xe-129 Magnetofluorescent Biosensor".ANALYTICAL CHEMISTRY 89.4(2017):2288-2295. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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