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Pseudo-typed Semliki Forest virus delivers EGFP into neurons

文献类型：期刊论文

作者Miao, Huan2; Xu, Fuqiang1,2,3,4; Zhu, Xutao3; Jia, Fan1,2
刊名JOURNAL OF NEUROVIROLOGY
出版日期2017-04-01
卷号23期号:2页码:205-215
关键词Semliki Forest Virus Replication-competent Vlps Packaging Sequence Pseudo-typed Sfv Vsvg Rvg
DOI10.1007/s13365-016-0486-8
文献子类Article
英文摘要Semliki Forest virus (SFV), a neurotropic virus, has been used to deliver heterologous genes into cells in vitro and in vivo. In this study, we constructed a reporter SFV4-FL-EGFP and found that it can deliver EGFP into neurons located at the injection site without disseminating throughout the brain. Lacking of the capsid gene of SFV4-FL-EGFP does not block its life cycle, while forming replication-competent virus-like particles (VLPs). These VLPs hold subviral genome by using the packaging sequence (PS) located within the nsP2 gene, and can transfer their genome into cells. In addition, we found that the G protein of vesicular stomatitis virus (VSVG) can package SFV subviral genome, which is consistent with the previous reports. The G protein of rabies virus (RVG) could also package SFV subviral genome. These pseudo-typed SFV can deliver EGFP gene into neurons. Taken together, these findings may be used to construct various SFV-based delivery systems for virological studies, gene therapy, and neural circuit labeling.
WOS关键词NONSTRUCTURAL PROTEIN NSP2 ; TICK-BORNE ENCEPHALITIS ; CORE-LIKE PARTICLES ; SINDBIS VIRUS ; A7(74) STRAIN ; VIRAL GENOME ; GENE-THERAPY ; RNA ; VECTORS ; SINGLE
WOS研究方向Neurosciences & Neurology ; Virology
语种英语
WOS记录号WOS:000396214900003
资助机构National Natural Science Foundation of China(31400975 ; National Natural Science Foundation of China(31400975 ; National Basic Research Program (973 Program) of China(2015CB755600) ; National Basic Research Program (973 Program) of China(2015CB755600) ; Strategic Priority Research Program (B)(XDB02050500) ; Strategic Priority Research Program (B)(XDB02050500) ; 91132307/H09) ; 91132307/H09) ; National Natural Science Foundation of China(31400975 ; National Natural Science Foundation of China(31400975 ; National Basic Research Program (973 Program) of China(2015CB755600) ; National Basic Research Program (973 Program) of China(2015CB755600) ; Strategic Priority Research Program (B)(XDB02050500) ; Strategic Priority Research Program (B)(XDB02050500) ; 91132307/H09) ; 91132307/H09)
源URL[http://ir.wipm.ac.cn/handle/112942/10880]  
专题武汉物理与数学研究所_磁共振应用研究部
作者单位1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, Wuhan 430071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714		 Miao, Huan,Xu, Fuqiang,Zhu, Xutao,et al. Pseudo-typed Semliki Forest virus delivers EGFP into neurons[J]. JOURNAL OF NEUROVIROLOGY,2017,23(2):205-215.
APA Miao, Huan,Xu, Fuqiang,Zhu, Xutao,&Jia, Fan.(2017).Pseudo-typed Semliki Forest virus delivers EGFP into neurons.JOURNAL OF NEUROVIROLOGY,23(2),205-215.
MLA Miao, Huan,et al."Pseudo-typed Semliki Forest virus delivers EGFP into neurons".JOURNAL OF NEUROVIROLOGY 23.2(2017):205-215.

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来源：武汉物理与数学研究所

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