Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease
文献类型：期刊论文
| 作者 | Chen, Yanke1; Xie, Hao2; Yang, Jun1; Li, Qichang2 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017-03-01 |
| 卷号 | 181期号:3页码:939-947 |
| 关键词 | Tagremoval Stericocclusions Maltosebinding Protein Integral Membrane Protein Expression And Purification Tev Protease |
| DOI | 10.1007/s12010-016-2260-z |
| 文献子类 | Article |
| 英文摘要 | Tag removal is a prerequisite issue for structural and functional analysis of affinity-purified membrane proteins. The present study took a MBP-fused membrane protein, MrpF, as a model to investigate the tag removal by TEV protease. Influences of the linking sequence between TEV cleavage site and MrpF on protein expression and predicted secondary structure were investigated. The steric accessibility of TEV protease to cleavage site of MBP-fused MrpF was explored. It was found that reducing the size of hydrophilic group of detergents and/or extending the linking sequence between cleavage site and target protein can significantly improve the accessibility of the cleavage site and promote tag removal by TEV protease. |
| WOS关键词 | ETCH VIRUS PROTEASE ; ESCHERICHIA-COLI ; AFFINITY TAGS ; DETERGENT SOLUTIONS ; BACILLUS-SUBTILIS ; PURIFICATION ; EXPRESSION ; STRATEGIES ; TOPOLOGY ; GENOMES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000394992900006 |
| 资助机构 | Ministry of Science and Technology(2016YFA0501200) ; Ministry of Science and Technology(2016YFA0501200) ; National Natural Science Foundation of China(31500603 ; National Natural Science Foundation of China(31500603 ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; 21425523) ; 21425523) ; Ministry of Science and Technology(2016YFA0501200) ; Ministry of Science and Technology(2016YFA0501200) ; National Natural Science Foundation of China(31500603 ; National Natural Science Foundation of China(31500603 ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; 21425523) ; 21425523) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/10888]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yanke,Xie, Hao,Yang, Jun,et al. Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,181(3):939-947. |
| APA | Chen, Yanke,Xie, Hao,Yang, Jun,&Li, Qichang.(2017).Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,181(3),939-947. |
| MLA | Chen, Yanke,et al."Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 181.3(2017):939-947. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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