Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation
文献类型：期刊论文
| 作者 | Wang, Huapu1,2; Zhu, Jiang2; Yang, Yunhuang2; Liu, Maili2; Kennedy, Michael A.3,4; Ramelot, Theresa A.3,4; Yue, Xiali1; Hu, Rui2 |
| 刊名 | PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 85期号:5页码:957-962 |
| 关键词 | Insm1 Zf4-5 Consensus Dna Solution Structure Mechanism |
| DOI | 10.1002/prot.25259 |
| 文献子类 | Article |
| 英文摘要 | Human INSM1 containing five C-terminal C2H2-type zinc fingers (ZFs), is a key regulator of neuroendocrine development. Previous research reported that full-length INSM1 containing all five ZFs recognized a consensus DNA sequence. Structure elucidation of human INSM1 ZFs is currently insufficient to understand the DNA binding mechanism. Herein, we present the solution NMR structure of ZF4-5, in which the two ZFs adopt a head-to-tail arrangement and each ZF features a canonical fold. NMR titrations and isothermal titration calorimetry experiments showed that ZF4-5 binds weakly to the consensus DNA sequence. Proteins 2017; 85:957-962. (c) 2016 Wiley Periodicals, Inc. |
| WOS关键词 | DNA-BINDING DOMAIN ; PROTEIN STRUCTURES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000399380400015 |
| 资助机构 | National Institute of General Medical Sciences of USA(U54-GM074958 ; National Institute of General Medical Sciences of USA(U54-GM074958 ; Ministry of Science and Technology(2016YFA051201) ; Ministry of Science and Technology(2016YFA051201) ; National Natural Sciences Foundation of China(21575155) ; National Natural Sciences Foundation of China(21575155) ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; U54-GM094597) ; U54-GM094597) ; National Institute of General Medical Sciences of USA(U54-GM074958 ; National Institute of General Medical Sciences of USA(U54-GM074958 ; Ministry of Science and Technology(2016YFA051201) ; Ministry of Science and Technology(2016YFA051201) ; National Natural Sciences Foundation of China(21575155) ; National Natural Sciences Foundation of China(21575155) ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; U54-GM094597) ; U54-GM094597) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11294]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Huazhong Agr Univ, Dept Chem, Coll Sci, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 3.Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA 4.Miami Univ, Northeast Struct Genom Consortium, Oxford, OH 45056 USA |
| 推荐引用方式 GB/T 7714 | Wang, Huapu,Zhu, Jiang,Yang, Yunhuang,et al. Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,2017,85(5):957-962. |
| APA | Wang, Huapu.,Zhu, Jiang.,Yang, Yunhuang.,Liu, Maili.,Kennedy, Michael A..,...&Hu, Rui.(2017).Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation.PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,85(5),957-962. |
| MLA | Wang, Huapu,et al."Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation".PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 85.5(2017):957-962. |
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来源：武汉物理与数学研究所
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