中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
  4. 磁共振基础研究部
The Effects of Macromolecular Crowding on Calmodulin Structure and Function

文献类型：期刊论文

作者Liu, Maili1; Li, Conggang1; Liu, Xiaoli1; Wu, Qiong1; Cheng, Kai1,2; Zhao, Jiajing1,2; Xu, Guohua1
刊名CHEMISTRY-A EUROPEAN JOURNAL
出版日期2017-05-01
卷号23期号:28页码:6736-6740
关键词Calmodulin Macromolecular Crowding Nmr Spectroscopy Protein Structure And Function
DOI10.1002/chem.201700367
文献子类Article
英文摘要Macromolecular crowding and confinement are two factors that potentially affect protein structure and function in a complex cellular environment. The confinement effect on the structure and function of holoCaM [Ca2+-loaded calmodulin (CaM)], a two-domain protein involved in many calcium-mediated signaling pathways, has been investigated previously. However, little is known about how macromolecular crowding affects holoCaM structure and function. Here, the structure-function correlations of holoCaM are investigated in macromolecular crowded environments. It was found that macromolecular crowding impacts its structure and function mildly. The major conformational states are still extended conformation with inter-domain separation in crowded environment as well as those in dilute solution, but the population of transiently compact conformation increases compared to dilute solution. Furthermore, macromolecular crowding facilitates the binding of CaM with AcN19 peptide (CaM-bind domain of -syn). This study provides a direct comparison for macromolecular crowding and confinement effects on protein structure and function, which helps to understand chemistry regulation in living cells.
WOS关键词PARAMAGNETIC RELAXATION ENHANCEMENT ; ALPHA-SYNUCLEIN ; BIOLOGICAL CONSEQUENCES ; PROTEIN ; BINDING ; STABILITY ; ENVIRONMENTS ; CONFINEMENT ; TRANSIENT ; STATES
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000401573100006
资助机构Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21173258 ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21173258 ; K. C. Wong Education Foundation ; K. C. Wong Education Foundation ; 21575156 ; 21575156 ; 21505152) ; 21505152) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21173258 ; 1000 Young Talents Program, National Natural Sciences Foundation of China(21173258 ; K. C. Wong Education Foundation ; K. C. Wong Education Foundation ; 21575156 ; 21575156 ; 21505152) ; 21505152)
源URL[http://ir.wipm.ac.cn/handle/112942/11307]  
专题武汉物理与数学研究所_磁共振基础研究部
作者单位1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Maili,Li, Conggang,Liu, Xiaoli,et al. The Effects of Macromolecular Crowding on Calmodulin Structure and Function[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017,23(28):6736-6740.
APA Liu, Maili.,Li, Conggang.,Liu, Xiaoli.,Wu, Qiong.,Cheng, Kai.,...&Xu, Guohua.(2017).The Effects of Macromolecular Crowding on Calmodulin Structure and Function.CHEMISTRY-A EUROPEAN JOURNAL,23(28),6736-6740.
MLA Liu, Maili,et al."The Effects of Macromolecular Crowding on Calmodulin Structure and Function".CHEMISTRY-A EUROPEAN JOURNAL 23.28(2017):6736-6740.

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。