Lanthanoid tagging via an unnatural amino acid for protein structure characterization
文献类型：期刊论文
| 作者 | Gu, Xin-Hua2; Tang, Chun1,2; Dong, Xu2; Jiang, Wen-Xue1,2 |
| 刊名 | JOURNAL OF BIOMOLECULAR NMR
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 67期号:4页码:273-282 |
| 关键词 | Unnatural Amino Acid Azide-alkyne Cycloaddition Pseudo-contact Shift Protein Structure Transient Protein Complex |
| DOI | 10.1007/s10858-017-0106-9 |
| 文献子类 | Article |
| 英文摘要 | Lanthanoid pseudo-contact shift (PCS) provides long-range structural information between a paramagnetic tag and protein nuclei. However, for proteins with native cysteines, site-specific attachment may only utilize functional groups orthogonal to sulfhydryl chemistry. Here we report two lanthanoid probes, DTTA-C3-yne and DTTA-C4-yne, which can be conjugated to an unnatural amino acid pAzF in the target protein via azide-alkyne cycloaddition. Demonstrated with ubiquitin and cysteine-containing enzyme EIIB, we show that large PCSs of distinct profiles can be generated for each tag/lanthanoid combination. The DTTA-based lanthanoid tags are associated with large magnetic susceptibility tensors owing to the rigidity of the tags. In particular, introduction of the DTTA-C3 tag affords intermolecular PCSs and enables structural characterization of a transient protein complex between ubiquitin and a UBA domain. Together, we have expanded the repertoire of paramagnetic tags and the applicability of paramagnetic NMR. |
| WOS关键词 | PARAMAGNETIC RELAXATION ENHANCEMENT ; PSEUDOCONTACT SHIFTS ; PHOSPHOTRANSFERASE SYSTEM ; ESCHERICHIA-COLI ; NMR-SPECTROSCOPY ; WEAK ALIGNMENT ; GENETIC-CODE ; UBA DOMAIN ; TAGS ; COMPLEXES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000400891000002 |
| 资助机构 | Chinese Ministry of Science and Technology(2013CB910200 ; Chinese Ministry of Science and Technology(2013CB910200 ; National Natural Science Foundation of China(31225007 ; National Natural Science Foundation of China(31225007 ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Howard Hughes Medical Institute ; Howard Hughes Medical Institute ; 2016YFA0501200) ; 2016YFA0501200) ; 31400644) ; 31400644) ; Chinese Ministry of Science and Technology(2013CB910200 ; Chinese Ministry of Science and Technology(2013CB910200 ; National Natural Science Foundation of China(31225007 ; National Natural Science Foundation of China(31225007 ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Howard Hughes Medical Institute ; Howard Hughes Medical Institute ; 2016YFA0501200) ; 2016YFA0501200) ; 31400644) ; 31400644) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11313]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Collaborat Innova, Wuhan Inst Phys & Math,State Key Lab Magnet Reson, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Xin-Hua,Tang, Chun,Dong, Xu,et al. Lanthanoid tagging via an unnatural amino acid for protein structure characterization[J]. JOURNAL OF BIOMOLECULAR NMR,2017,67(4):273-282. |
| APA | Gu, Xin-Hua,Tang, Chun,Dong, Xu,&Jiang, Wen-Xue.(2017).Lanthanoid tagging via an unnatural amino acid for protein structure characterization.JOURNAL OF BIOMOLECULAR NMR,67(4),273-282. |
| MLA | Gu, Xin-Hua,et al."Lanthanoid tagging via an unnatural amino acid for protein structure characterization".JOURNAL OF BIOMOLECULAR NMR 67.4(2017):273-282. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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