An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain
文献类型：期刊论文
| 作者 | Qu, Bo1,3; Wu, Ai-Bo3,5; Guo, Mao-Wei1,3; Yuan, Qing-Song1,3; Tang, Xue-Ling1,3; Yi, Shu-Yuan1,3; Zhang, Limin6; He, Wei-Jie2,3; Li, He-Ping2,3; Liao, Yu-Cai1,3,4 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-08-25 |
| 卷号 | 7 |
| DOI | 10.1038/s41598-017-08799-w |
| 文献子类 | Article |
| 英文摘要 | Degradation of toxins by microorganisms is a promising approach for detoxification of agricultural products. Here, a bacterial strain, Sphingomonas S3-4, that has the ability to degrade the mycotoxin deoxynivalenol (DON) was isolated from wheat fields. Incubation of Fusarium-infected wheat grains with S3-4 completely eliminated DON. In S3-4 DON is catabolized into compounds with no detectable phytotoxicity, 3-oxo-DON and 3-epi-DON, via two sequential reactions. Comparative analysis of genome sequences from two DON-degrading strains, S3-4 and Devosia D17, and one non-DON-degrading strain, Sphingobium S26, combined with functional screening of a S3-4 genomic BAC library led to the discovery that a novel aldo/keto reductase superfamily member, AKR18A1, is responsible for oxidation of DON into 3-oxo-DON. DON-degrading activity is completely abolished in a mutant S3-4 strain where the AKR18A1 gene is disrupted. Recombinant AKR18A1 protein expressed in Escherichia coli catalyzed the reversible oxidation/reduction of DON at a wide range of pH values (7.5 to 11) and temperatures (10 to 50 degrees C). The S3-4 strain and recombinant AKR18A1 also catabolized zearalenone and the aldehydes glyoxal and methyglyoxal. The S3-4 strain and the AKR18A1 gene are promising agents for the control of Fusarium pathogens and detoxification of mycotoxins in plants and in food/feed products. |
| WOS关键词 | HEAD BLIGHT ; MYCOTOXIN DEOXYNIVALENOL ; ESCHERICHIA-COLI ; RNA GENES ; DETOXIFICATION ; WHEAT ; DEGRADATION ; RESISTANCE ; SUPERFAMILY ; ANNOTATION |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000408449000028 |
| 资助机构 | National Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; National Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11368]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China 3.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China 4.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety, Res Inst Nutr Sci, Shanghai 200031, Peoples R China 6.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Bo,Wu, Ai-Bo,Guo, Mao-Wei,et al. An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Qu, Bo.,Wu, Ai-Bo.,Guo, Mao-Wei.,Yuan, Qing-Song.,Tang, Xue-Ling.,...&Liao, Yu-Cai.(2017).An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain.SCIENTIFIC REPORTS,7. |
| MLA | Qu, Bo,et al."An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：武汉物理与数学研究所
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