Fluorescence self-quenching assay for the detection of target collagen sequences using a short probe peptide
文献类型：期刊论文
| 作者 | Hu, Yue2; Fu, Caihong2; Nian, Linge2; Xiao, Jianxi1,2; Song, Chen2; Wang, Jie1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2018 |
| 卷号 | 176页码:492-498 |
| 关键词 | Collagen Fluorescence Self-quenching Peptide Targeting |
| DOI | 10.1016/j.talanta.2017.08.042 |
| 文献子类 | Article |
| 英文摘要 | The development of novel assays to detect collagen fragments is of utmost importance for diagnostic, prognostic and therapeutic decisions in various collagen-related diseases, and one essential question is to discover probe peptides that can specifically recognize target collagen sequences. Herein we have developed the fluorescence self-quenching assay as a convenient tool to screen the capability of a series of fluorescent probe peptides of variable lengths to bind with target collagen peptides. We have revealed that the targeting ability of probe peptides is length-dependent, and have discovered a relatively short probe peptide FAM-G(POG)(8) capable to identify the target peptide. We have further demonstrated that fluorescence self-quenching assay together with this short probe peptide can be applied to specifically detect the desired collagen fragment in complex biological media. Fluorescence self-quenching assay provides a powerful new tool to discover effective peptides for the recognition of collagen biomarkers, and it may have great potential to identify probe peptides for various protein biomarkers involved in pathological conditions. |
| WOS关键词 | MONOCLONAL-ANTIBODIES ; II COLLAGEN ; TRIPLE-HELIX ; MARKERS ; ANGIOGENESIS ; DEGRADATION ; STABILITY ; FIBROSIS ; DISEASES |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000412610100062 |
| 资助机构 | National Natural Science Foundation of China(21305056) ; National Natural Science Foundation of China(21305056) ; Fundamental Research Funds for the Central Universities(lzujbky-20160-k10) ; Fundamental Research Funds for the Central Universities(lzujbky-20160-k10) ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; National Natural Science Foundation of China(21305056) ; National Natural Science Foundation of China(21305056) ; Fundamental Research Funds for the Central Universities(lzujbky-20160-k10) ; Fundamental Research Funds for the Central Universities(lzujbky-20160-k10) ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11430]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan 430071, Hubei, Peoples R China 2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yue,Fu, Caihong,Nian, Linge,et al. Fluorescence self-quenching assay for the detection of target collagen sequences using a short probe peptide[J]. TALANTA,2018,176:492-498. |
| APA | Hu, Yue,Fu, Caihong,Nian, Linge,Xiao, Jianxi,Song, Chen,&Wang, Jie.(2018).Fluorescence self-quenching assay for the detection of target collagen sequences using a short probe peptide.TALANTA,176,492-498. |
| MLA | Hu, Yue,et al."Fluorescence self-quenching assay for the detection of target collagen sequences using a short probe peptide".TALANTA 176(2018):492-498. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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