Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandemmass Spectrometry
文献类型：期刊论文
| 作者 | Liu, Xin5; Hao, Fuhua4; Lei, Hehua4; Zhou, Lihong4,5; Wang, Jin1,3,4,5; Tang, Huiru1,3; Wang, Yulan2,4 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-05-03 |
| 卷号 | 7 |
| DOI | 10.1038/s41598-017-01435-7 |
| 文献子类 | Article |
| 英文摘要 | Metabolites containing amino groups cover multiple pathways and play important roles in redox homeostasis and biosyntheses of proteins, nucleotides and neurotransmitters. Here, we report a new method for simultaneous quantification of 124 such metabolites. This is achieved by derivatizationassisted sensitivity enhancement with 5-aminoisoquinolyl-N-hydroxysuccinimidyl carbamate (5AIQC) followed with comprehensive analysis using ultra-high performance liquid chromatography and electrospray ionization tandem mass spectrometry (UHPLC-MS/MS). In an one-pot manner, this quantification method enables simultaneous coverage of 20 important metabolic pathways including protein biosynthesis/degradation, biosyntheses of catecholamines, arginine and glutathione, metabolisms of homocysteine, taurine-hypotaurine etc. Compared with the reported ones, this method is capable of simultaneously quantifying thiols, disulfides and other oxidation-prone analytes in a single run and suitable for quantifying aromatic amino metabolites. This method is also much more sensitive for all tested metabolites with LODs well below 50 fmol (at sub-fmol for most tested analytes) and shows good precision for retention time and quantitation with inter-day and intra-day relative standard deviations (RSDs) below 15% and good recovery from renal cancer tissue, rat urine and plasma. The method was further applied to quantify the amino metabolites in silkworm hemolymph from multiple developmental stages showing its applicability in metabolomics and perhaps some clinical chemistry studies. |
| WOS关键词 | HUMAN COLORECTAL-CANCER ; HIGH-FAT DIET ; MASS-SPECTROMETRY ; METABONOMIC ANALYSIS ; SYSTEMS BIOLOGY ; ACID-ANALYSIS ; DIAGNOSIS ; MICE ; NMR ; PROFILES |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000400491400001 |
| 资助机构 | National Natural Science Foundation of China(91439102 ; National Natural Science Foundation of China(91439102 ; 81590953 ; 81590953 ; 21375144 ; 21375144 ; 21405020) ; 21405020) ; National Natural Science Foundation of China(91439102 ; National Natural Science Foundation of China(91439102 ; 81590953 ; 81590953 ; 21375144 ; 21375144 ; 21405020) ; 21405020) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11453]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Fudan Univ, Sch Life Sci, Shanghai Int Ctr Mol Phen, Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China 2.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China 3.Zhongshan Hosp, State Key Lab Genet Engn, Shanghai 200438, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, CAS Key Lab Magnet Resonance Biol Syst,State Key, Wuhan 430071, Peoples R China 5.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xin,Hao, Fuhua,Lei, Hehua,et al. Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandemmass Spectrometry[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Liu, Xin.,Hao, Fuhua.,Lei, Hehua.,Zhou, Lihong.,Wang, Jin.,...&Wang, Yulan.(2017).Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandemmass Spectrometry.SCIENTIFIC REPORTS,7. |
| MLA | Liu, Xin,et al."Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandemmass Spectrometry".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：武汉物理与数学研究所
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