Chemical reactivation of resin-embedded pHuji adds red for simultaneous two-color imaging with EGFP
文献类型：期刊论文
| 作者 | Zhou, Hongfu2,3; Guo, Wenyan2,3; Liu, Xiuli2,3; Liu, Yurong2,3; Gang, Yadong2,3; He, Xiaobin1; Jia, Yao2,3; Yin, Fangfang2,3; Li, Pei2,3; Huang, Fei2,3 |
| 刊名 | BIOMEDICAL OPTICS EXPRESS
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 8期号:7页码:3281-3288 |
| DOI | 10.1364/BOE.8.003281 |
| 文献子类 | Article |
| 英文摘要 | The pH-sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging. EGFP or EYFP is good for such applications. For simultaneous two-color imaging, a suitable red fluorescent protein is an urgent need. Here a pH-sensitive red fluorescent protein, pHuji, is selected and verified to remain pH-sensitive in HM20 resin. We observe 183% fluorescence intensity of pHuji in resin-embeded mouse brain and 29.08-fold fluorescence intensity of reactivated pHuji compared to the quenched state. pHuji and EGFP can be quenched and chemically reactivated simultaneously in resin, thus enabling simultaneous two-color micro-optical sectioning tomography of resin-embedded mouse brain. This method may greatly facilitate the visualization of neuronal morphology and neural circuits to promote understanding of the structure and function of the brain. (C) 2017 Optical Society of America |
| WOS关键词 | OPTICAL SECTIONING TOMOGRAPHY ; GREEN FLUORESCENT PROTEIN ; NEURAL CIRCUITS ; RABIES VIRUS ; MOUSE-BRAIN ; RESOLUTION ; ORANGE ; PH |
| WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000404738300009 |
| 资助机构 | Program 973(2015CB755603) ; Program 973(2015CB755603) ; Program 973(2015CB755603) ; Program 973(2015CB755603) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11463]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, State Key Lab Magnet Resonance & Atom & Mol Phys, West 30 Xiao Hong Shan, Wuhan 430071, Peoples R China 2.Huazhong Univ Sci & Technol, Dept Biomed Engn, Key Lab Biomed Photon, Minist Educ, 1037 Luoyu Rd, Wuhan 430074, Peoples R China 3.Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, 1037 Luoyu Rd, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Hongfu,Guo, Wenyan,Liu, Xiuli,et al. Chemical reactivation of resin-embedded pHuji adds red for simultaneous two-color imaging with EGFP[J]. BIOMEDICAL OPTICS EXPRESS,2017,8(7):3281-3288. |
| APA | Zhou, Hongfu.,Guo, Wenyan.,Liu, Xiuli.,Liu, Yurong.,Gang, Yadong.,...&Zeng, Shaoqun.(2017).Chemical reactivation of resin-embedded pHuji adds red for simultaneous two-color imaging with EGFP.BIOMEDICAL OPTICS EXPRESS,8(7),3281-3288. |
| MLA | Zhou, Hongfu,et al."Chemical reactivation of resin-embedded pHuji adds red for simultaneous two-color imaging with EGFP".BIOMEDICAL OPTICS EXPRESS 8.7(2017):3281-3288. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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