Fluorescence characterization of the thermal stability of collagen mimic peptides
文献类型：期刊论文
| 作者 | Xiao, Jian-Xi1,2; Sun, Xiu-Xia1,2; Fan, Jun1; Hou, Yan-Nan1; Liang, Shuo1; Zhang, Yu-Ping1 |
| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 28期号:5页码:963-967 |
| 关键词 | Collagen Peptides Thermal Stability Fluorescence Triple Helix |
| DOI | 10.1016/j.cclet.2016.11.029 |
| 文献子类 | Article |
| 英文摘要 | The thermal stability of triple helical structure plays a critical role in collagen biosynthesis, function and degradation. CD technique was utilized to characterize the thermal stability of synthetic collagen mimic peptides. Fluorescence spectroscopy is widely used with easy access all around the world because of its inexpensive instrumentation, low operation cost, easy operation, and high sensitivity. Here we have developed an alternative fluorescence method to detect the thermal stability of collagen mimic peptides. We have demonstrated that fluorescence spectroscopy could measure the thermal stability of collagen mimic peptides with low concentrations under different circumstances. This highly sensitive fluorescence self-quenching assay will greatly expedite the studies of sequence-dependent properties of collagen mimic peptides, and it has great potential in the application of determining the thermal stability of triple helix systems such as collagens, collectins, adiponectin, macrophage scavenger and C1q. (C) 2016 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved. |
| WOS关键词 | TRIPLE-HELIX ; OSTEOGENESIS IMPERFECTA ; CONFORMATIONAL STABILITY ; I COLLAGEN ; MODEL |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000402444600008 |
| 资助机构 | National Natural Science Foundation of China(21305056) ; National Natural Science Foundation of China(21305056) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; National Natural Science Foundation of China(21305056) ; National Natural Science Foundation of China(21305056) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11495]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Jian-Xi,Sun, Xiu-Xia,Fan, Jun,et al. Fluorescence characterization of the thermal stability of collagen mimic peptides[J]. CHINESE CHEMICAL LETTERS,2017,28(5):963-967. |
| APA | Xiao, Jian-Xi,Sun, Xiu-Xia,Fan, Jun,Hou, Yan-Nan,Liang, Shuo,&Zhang, Yu-Ping.(2017).Fluorescence characterization of the thermal stability of collagen mimic peptides.CHINESE CHEMICAL LETTERS,28(5),963-967. |
| MLA | Xiao, Jian-Xi,et al."Fluorescence characterization of the thermal stability of collagen mimic peptides".CHINESE CHEMICAL LETTERS 28.5(2017):963-967. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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