Characterization of the in vitro assembly of FtsZ in Arthrobacter strain A3 using light scattering
文献类型：期刊论文
| 作者 | Hou, Yannan1; Yu, Linghui4; Chen, Ximing2; Xiao, Jianxi1,3; Yang, Fan1,4; Zhang, Shanshan1; Ding, Shenglong1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 91页码:294-298 |
| 关键词 | Ftsz Light Scattering Polymerization In Vitro Assembly Arthrobacter Strain A3 Cell Division |
| DOI | 10.1016/j.ijbiomac.2016.04.090 |
| 文献子类 | Article |
| 英文摘要 | The self-assembly of FtsZ, the bacterial homolog of tubulin, plays an essential role in cell division. Light scattering technique is applied to real-time monitor the in vitro assembly of FtsZ in Arthrobacter strain A3, a newly isolated psychrotrophic bacterium. The critical concentration needed for the assembly is estimated as 6.7 mu M. The polymerization of FtsZ in Arthrobacter strain A3 requires both GTP and divalent metal ions, while salt is an unfavorable condition for the assembly. The FtsZ polymerizes under a wide range of pHs, with the fastest rate around pH 6.0. The FtsZ from Arthrobacter strain A3 resembles Mycobacterium tuberculosis FtsZ in terms of the dependence on divalent metal ions and the slow polymerization rate, while it is different from M. tuberculosis FtsZ considering the sensitivity to salt and pH. The comparison of FtsZ from different organisms will greatly advance our understanding of the biological role of the key cell division protein. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | CELL DIVISION PROTEIN ; MYCOBACTERIUM-TUBERCULOSIS FTSZ ; ESCHERICHIA-COLI ; POLYMERIZATION ; GTP ; CAULOBACTER ; CYTOSKELETON ; TUBULIN ; FORM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000382339200034 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11505]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Desert & Desertificat, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 4.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Yannan,Yu, Linghui,Chen, Ximing,et al. Characterization of the in vitro assembly of FtsZ in Arthrobacter strain A3 using light scattering[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,91:294-298. |
| APA | Hou, Yannan.,Yu, Linghui.,Chen, Ximing.,Xiao, Jianxi.,Yang, Fan.,...&Ding, Shenglong.(2016).Characterization of the in vitro assembly of FtsZ in Arthrobacter strain A3 using light scattering.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,91,294-298. |
| MLA | Hou, Yannan,et al."Characterization of the in vitro assembly of FtsZ in Arthrobacter strain A3 using light scattering".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 91(2016):294-298. |
入库方式： OAI收割
来源：武汉物理与数学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。