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Measurement of amide proton chemical shift anisotropy in perdeuterated proteins using CSA amplification

文献类型：期刊论文

作者Hung, Ivan1; Gan, Zhehong1; Li, Conggang2; Liu, Xiaoli2; Ge, Yuwei1,2,3; Liu, Maili2
刊名JOURNAL OF MAGNETIC RESONANCE
出版日期2017-11-01
卷号284页码:33-38
关键词Chemical Shift Anisotropy Magic-angle Spinning Spinning Sidebands Csa Amplification Xcsa Gb1
DOI10.1016/j.jmr.2017.09.009
文献子类Article
英文摘要Measuring H-1 chemical shift anisotropy (CSA) is useful for probing proton environments and dynamics but remains a challenge due to strong homonuclear interaction and relatively small shift anisotropy, especially in proteins with multiple proton sites. Here the extended chemical shift anisotropy amplification (xCSA) method is applied for amide proton CSA measurement in uniformly H-2 enriched proteins under fast magic angle spinning. The xCSA method is capable of distinguishing the sign of the CSA asymmetry parameter, complimenting other multiple-pulse recoupling methods. A three-dimensional xCSA experiment is demonstrated for measuring the proton CSA of amide sites in a GB1 protein sample and the possible correlation of amide proton CSA with protein secondary structure is discussed. (C) 2017 Elsevier Inc. All rights reserved.
WOS关键词SOLID-STATE NMR ; NUCLEAR-MAGNETIC-RESONANCE ; ANGLE-SPINNING NMR ; CROSS-CORRELATED RELAXATION ; PULSE SEQUENCES ; MICROCRYSTALLINE PROTEIN ; ROTATING SOLIDS ; SPECTROSCOPY ; CARBONYL ; MAS
WOS研究方向Biochemistry & Molecular Biology ; Physics ; Spectroscopy
语种英语
WOS记录号WOS:000414889800005
资助机构Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; National Natural Science Foundation of China(21575156) ; National Natural Science Foundation of China(21575156) ; K.C.Wong Education Foundation ; K.C.Wong Education Foundation ; National High Magnetic Field Laboratory, the State of Florida(NSF DMR-1157490) ; National High Magnetic Field Laboratory, the State of Florida(NSF DMR-1157490) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; National Natural Science Foundation of China(21575156) ; National Natural Science Foundation of China(21575156) ; K.C.Wong Education Foundation ; K.C.Wong Education Foundation ; National High Magnetic Field Laboratory, the State of Florida(NSF DMR-1157490) ; National High Magnetic Field Laboratory, the State of Florida(NSF DMR-1157490)
源URL[http://ir.wipm.ac.cn/handle/112942/11553]  
专题武汉物理与数学研究所_磁共振基础研究部
作者单位1.Natl High Magnet Field Lab, Ctr Interdisciplinary Magnet Resonance, 1800 East Paul Dirac Dr, Tallahassee, FL 32310 USA
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049+, Peoples R China
推荐引用方式
GB/T 7714		 Hung, Ivan,Gan, Zhehong,Li, Conggang,et al. Measurement of amide proton chemical shift anisotropy in perdeuterated proteins using CSA amplification[J]. JOURNAL OF MAGNETIC RESONANCE,2017,284:33-38.
APA Hung, Ivan,Gan, Zhehong,Li, Conggang,Liu, Xiaoli,Ge, Yuwei,&Liu, Maili.(2017).Measurement of amide proton chemical shift anisotropy in perdeuterated proteins using CSA amplification.JOURNAL OF MAGNETIC RESONANCE,284,33-38.
MLA Hung, Ivan,et al."Measurement of amide proton chemical shift anisotropy in perdeuterated proteins using CSA amplification".JOURNAL OF MAGNETIC RESONANCE 284(2017):33-38.

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来源：武汉物理与数学研究所

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