Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe
文献类型：期刊论文
| 作者 | Liu, Maili1; Liu, Hongbin1; Chen, Shizhen1; Yang, Shengjun1; Guo, Qianni1; Zeng, Qingbin1; Zhou, Xin1; McMahon, Michael T.2,3 |
| 刊名 | TALANTA
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 184页码:513-519 |
| 关键词 | F-19 Nuclear Magnetic Resonance Biothiols Probes Thiol-addition/intramolecular Cyclization |
| DOI | 10.1016/j.talanta.2018.03.039 |
| 文献子类 | Article |
| 英文摘要 | Simultaneous detection and differentiation of biomolecules is of significance in biological research. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. However, existing methods for simultaneous detection and differentiation of Cys, Hcy, and GSH are still challenging because of their similarity in structure and chemical properties. Herein we report a probe that simultaneously detects and discriminates between mixtures of Cys, Hcy and GSH using F-19 nuclear magnetic resonance (NMR). This F-19 NMR probe responds rapidly to biothiols through the Michael addition reaction and subsequent intramolecular cyclization reaction allowing differentiation between Cys, Hcy and GSH through F-19 NMR chemical shift. We demonstrate that this F-19 NMR probe is a powerful method for analysis of complex mixtures. |
| WOS关键词 | HIGHLY SELECTIVE DETECTION ; MASS-SPECTROMETRY ; FLUORESCENT-PROBE ; REACTIVE OXYGEN ; CONTRAST AGENTS ; LIVING CELLS ; IN-VIVO ; CYSTEINE ; GLUTATHIONE ; MRI |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000432234900066 |
| 资助机构 | National Natural Science Foundation of China(81625011 ; National Natural Science Foundation of China(81625011 ; National Key R&D Program of China(2016YFC1304700 ; National Key R&D Program of China(2016YFC1304700 ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; 81227902) ; 81227902) ; 2017YFA0505400) ; 2017YFA0505400) ; National Natural Science Foundation of China(81625011 ; National Natural Science Foundation of China(81625011 ; National Key R&D Program of China(2016YFC1304700 ; National Key R&D Program of China(2016YFC1304700 ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; 81227902) ; 81227902) ; 2017YFA0505400) ; 2017YFA0505400) |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/11984]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math,Key Lab Magnet Resonance B, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 2.Kennedy Krieger Inst, FM Kirby Res Ctr Funct Brain Imaging, Baltimore, MD 21205 USA 3.Johns Hopkins Sch Med, Dept Radiol & Radiol Sci, Baltimore, MD 21205 USA |
| 推荐引用方式 GB/T 7714 | Liu, Maili,Liu, Hongbin,Chen, Shizhen,et al. Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe[J]. TALANTA,2018,184:513-519. |
| APA | Liu, Maili.,Liu, Hongbin.,Chen, Shizhen.,Yang, Shengjun.,Guo, Qianni.,...&McMahon, Michael T..(2018).Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe.TALANTA,184,513-519. |
| MLA | Liu, Maili,et al."Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe".TALANTA 184(2018):513-519. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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