中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases

文献类型：期刊论文

作者Li, Conggang1; Liu, Maili1; Zhou, Pei4; Jiang, Ling1; Liu, Yixiang1; Rose, Joshua4; Huang, Shaojia2,3; Hu, Yangbo3; Wu, Qiong1; Wang, Dan1,2
刊名NATURE COMMUNICATIONS
出版日期2017-12-13
卷号8
ISSN号2041-1723
DOI10.1038/s41467-017-02310-9
文献子类Article
英文摘要Histidine kinases are key regulators in the bacterial two-component systems that mediate the cellular response to environmental changes. The vast majority of the sensor histidine kinases belong to the bifunctional HisKA family, displaying both kinase and phosphatase activities toward their substrates. The molecular mechanisms regulating the opposing activities of these enzymes are not well understood. Through a combined NMR and crystallographic study on the histidine kinase HK853 and its response regulator RR468 from Thermotoga maritima, here we report a pH-mediated conformational switch of HK853 that shuts off its phosphatase activity under acidic conditions. Such a pH-sensing mechanism is further demonstrated in the EnvZ-OmpR two-component system from Salmonella enterica in vitro and in vivo, which directly contributes to the bacterial infectivity. Our finding reveals a broadly conserved mechanism that regulates the phosphatase activity of the largest family of bifunctional histidine kinases in response to the change of environmental pH.
WOS关键词2-COMPONENT SIGNAL-TRANSDUCTION ; NMR-SPECTROSCOPY ; F-19 NMR ; ESCHERICHIA-COLI ; SSRA-SSRB ; SYSTEM ; OMPR ; EXCHANGE ; ENVZ ; PHOSPHORYLATION
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000417884500010
出版者NATURE PUBLISHING GROUP
资助机构U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268)
源URL[http://ir.wipm.ac.cn/handle/112942/12762]  
专题中国科学院武汉物理与数学研究所
通讯作者Jiang, Ling
作者单位1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst,Natl Ctr Magne, Wuhan 430071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China
4.Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
推荐引用方式
GB/T 7714		 Li, Conggang,Liu, Maili,Zhou, Pei,et al. A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases[J]. NATURE COMMUNICATIONS,2017,8.
APA Li, Conggang.,Liu, Maili.,Zhou, Pei.,Jiang, Ling.,Liu, Yixiang.,...&Wang, Dan.(2017).A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases.NATURE COMMUNICATIONS,8.
MLA Li, Conggang,et al."A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases".NATURE COMMUNICATIONS 8(2017).

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。