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A robust heteronuclear dipolar recoupling method comparable to TEDOR for proteins in magic-angle spinning solid-state NMR

文献类型：期刊论文

作者Zhang, Zhengfeng1; Yang, Jun1; Xie, Huayong1,2; Chen, Yanke1; Li, Jianping1
刊名JOURNAL OF MAGNETIC RESONANCE
出版日期2017-12-01
卷号285页码:79-85
关键词Broadband Dual Back-to-back Pulses (Bro-dbp) Heteronuclear Dipolar Recoupling Magic-angle Spinning (Mas) Solid-state Nmr Transferred-echo Double-resonance (Tedor) Proteins
ISSN号1090-7807
DOI10.1016/j.jmr.2017.10.012
文献子类Article
英文摘要In this letter, we propose a robust heteronuclear dipolar recoupling method for proteins in magic-angle spinning (MAS) solid-state NMR. This method is as simple, robust and efficient as the well-known TEDOR in the aspect of magnetization transfer between N-15 and C-13. Deriving from our recent band-selective dual back-to-back pulses (DBP) (Zhang et al., 2016), this method uses new phase-cycling schemes to realize broadband DBP (Bro-DBP). For broadband N-15-C-13 magnetization transfer (simultaneous N-15 -> C-13' and N-15 -> C-13 alpha), Bro-DBP has almost the same N-15 -> C-13 alpha efficiency while offers 30-40% enhancement on N-15 -> C-13' transfer, compared to TEDOR. Besides, Bro-DBP can also be used as a carbonyl (C-13') selected method, whose N-15 -> C-13' efficiency is up to 1.7 times that of TEDOR and is also higher than that of band-selective DBP. The performance of Bro-DBP is demonstrated on the N-formyl-[U-C-13, N-15]-Met-Leu-Phe-OH (fMLF) peptide and the U-C-13, N-15 labeled beta 1 immunoglobulin binding domain of protein G (GB1) microcrystalline protein. Since Bro-DBP is as robust, simple and efficient as TEDOR, we believe it is very useful for protein studies in MAS solid-state NMR. (C) 2017 Elsevier Inc. All rights reserved.
WOS关键词NUCLEAR-MAGNETIC-RESONANCE ; BETA-AMYLOID FIBRILS ; DOUBLE-CROSS-POLARIZATION ; ECHO DOUBLE-RESONANCE ; ROTATING SOLIDS ; REDOR NMR ; DISTANCE MEASUREMENTS ; COHERENCE TRANSFER ; UNIFORMLY C-13 ; DILUTE SPINS
WOS研究方向Biochemistry & Molecular Biology ; Physics ; Spectroscopy
语种英语
WOS记录号WOS:000418312800010
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
资助机构National Natural Science Foundation of China(21405171 ; National Natural Science Foundation of China(21405171 ; National Key R&D Program of China(2016YFA0501200 ; National Key R&D Program of China(2016YFA0501200 ; 21775161 ; 21775161 ; 2017YFA0505400) ; 2017YFA0505400) ; 21425523 ; 21425523 ; 21605156 ; 21605156 ; 31500603 ; 31500603 ; 31627803) ; 31627803) ; National Natural Science Foundation of China(21405171 ; National Natural Science Foundation of China(21405171 ; National Key R&D Program of China(2016YFA0501200 ; National Key R&D Program of China(2016YFA0501200 ; 21775161 ; 21775161 ; 2017YFA0505400) ; 2017YFA0505400) ; 21425523 ; 21425523 ; 21605156 ; 21605156 ; 31500603 ; 31500603 ; 31627803) ; 31627803) ; National Natural Science Foundation of China(21405171 ; National Natural Science Foundation of China(21405171 ; National Key R&D Program of China(2016YFA0501200 ; National Key R&D Program of China(2016YFA0501200 ; 21775161 ; 21775161 ; 2017YFA0505400) ; 2017YFA0505400) ; 21425523 ; 21425523 ; 21605156 ; 21605156 ; 31500603 ; 31500603 ; 31627803) ; 31627803) ; National Natural Science Foundation of China(21405171 ; National Natural Science Foundation of China(21405171 ; National Key R&D Program of China(2016YFA0501200 ; National Key R&D Program of China(2016YFA0501200 ; 21775161 ; 21775161 ; 2017YFA0505400) ; 2017YFA0505400) ; 21425523 ; 21425523 ; 21605156 ; 21605156 ; 31500603 ; 31500603 ; 31627803) ; 31627803)
源URL[http://ir.wipm.ac.cn/handle/112942/12772]  
专题中国科学院武汉物理与数学研究所
通讯作者Zhang, Zhengfeng; Yang, Jun
作者单位1.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst,Wuhan Inst Phy, Wuhan 430071, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Zhengfeng,Yang, Jun,Xie, Huayong,et al. A robust heteronuclear dipolar recoupling method comparable to TEDOR for proteins in magic-angle spinning solid-state NMR[J]. JOURNAL OF MAGNETIC RESONANCE,2017,285:79-85.
APA Zhang, Zhengfeng,Yang, Jun,Xie, Huayong,Chen, Yanke,&Li, Jianping.(2017).A robust heteronuclear dipolar recoupling method comparable to TEDOR for proteins in magic-angle spinning solid-state NMR.JOURNAL OF MAGNETIC RESONANCE,285,79-85.
MLA Zhang, Zhengfeng,et al."A robust heteronuclear dipolar recoupling method comparable to TEDOR for proteins in magic-angle spinning solid-state NMR".JOURNAL OF MAGNETIC RESONANCE 285(2017):79-85.

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来源：武汉物理与数学研究所

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