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UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria

文献类型：期刊论文

作者Chen, Min3; Yin, Xiao-Yue4; Zhang, Zhong-Chun4; Shang, Jin-Long4; Qiu, Bao-Sheng4; Tang, Hui-Ru1,5; Yin, Yan-Chao4; Xu, Hai-Feng4; Feng, Jun-Li4; Wang, Kai4
刊名ENVIRONMENTAL MICROBIOLOGY
出版日期2018
卷号20期号:1页码:200-213
ISSN号1462-2912
DOI10.1111/1462-2920.13972
文献子类Article
英文摘要The small-molecule sunscreen compounds, mycosporine-like amino acids (MAAs), have strong ultraviolet (UV) absorption and can protect cyanobacteria against UV-B damage. However, the molecular mechanism underlying UV-B signaling and MAA chemical diversity remain largely unclear. Here, we identified a five-gene cluster for MAA biosynthesis in the solar radiation and desiccation tolerant cyanobacterium Nostoc flagelliforme. A LuxR family protein OrrA was identified as a positive UV-B responsive regulator binding to the promoter region of this gene cluster. OrrA functions as an activator mediating the UV-B induced MAA biosynthesis. Overexpression of orrA strengthened its UV-B tolerance during desiccation, and enhanced the photosynthetic recovery upon rehydration. Heterologous expression of this gene cluster in Anabaena PCC 7120 produces the same MAA as that in field samples of N. flagelliforme. The MAA structure is assigned as mycosporine-2-(4-deoxygadusolyl-ornithine) with a molecular weight of 756 Da, the structurally unique MAA compound reported to date. This MAA was catalyzed by mysD-mysC2-mysC1 encoding proteins from 4-deoxygadusol, which was synthesized through the catalysis of mysA-mysB products. Thus, we elucidated the transcriptional mechanism for a novel type MAA biosynthesis in solar radiation and desiccation tolerant cyanobacteria, which shed light on the identification of other components for UV-B signaling in cyanobacteria.
WOS关键词CHLOROGLOEOPSIS PCC 6912 ; AMINO-ACIDS ; NOSTOC-FLAGELLIFORME ; PHOTOSYSTEM-II ; MICROCYSTIS-AERUGINOSA ; ULTRAVIOLET-RADIATION ; ESCHERICHIA-COLI ; COMMUNE ; NITROGEN ; PROTEIN
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000419784100016
出版者WILEY
资助机构National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309) ; National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309) ; National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309) ; National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309)
源URL[http://ir.wipm.ac.cn/handle/112942/12778]  
专题中国科学院武汉物理与数学研究所
通讯作者Qiu, Bao-Sheng
作者单位1.Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200438, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
3.Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia
4.Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China
5.Fudan Univ, Zhongshan Hosp, Collaborat Innovat Ctr Genet & Dev, Minist Educ,Key Lab Contemporary Anthropol Metabo, Shanghai 200438, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Min,Yin, Xiao-Yue,Zhang, Zhong-Chun,et al. UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria[J]. ENVIRONMENTAL MICROBIOLOGY,2018,20(1):200-213.
APA Chen, Min.,Yin, Xiao-Yue.,Zhang, Zhong-Chun.,Shang, Jin-Long.,Qiu, Bao-Sheng.,...&Hao, Fu-Hua.(2018).UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria.ENVIRONMENTAL MICROBIOLOGY,20(1),200-213.
MLA Chen, Min,et al."UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria".ENVIRONMENTAL MICROBIOLOGY 20.1(2018):200-213.

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来源：武汉物理与数学研究所

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