中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures

文献类型：期刊论文

作者Li, Qiang1,6; Tan, Dan3; Wang, Jian-Hua3,4,5; Zhang, Xiaoyun1,6; Li, Maodong2; Lei, Xiaoguang1,6; Dong, Meng-Qiu3,4,5; Liu, Zhirong2; Tang, Chun7; Gong, Zhou7
刊名ANALYTICAL CHEMISTRY
出版日期2018-01-16
卷号90期号:2页码:1195-1201
ISSN号0003-2700
DOI10.1021/acs.analchem.7b03789
文献子类Article
英文摘要Chemical cross-linking coupled with mass spectrometry (CXMS) facilitates structural analysis, of proteins. As current CXMS applications are almost exclusively limitedto lysine residues, they Can only retrieve a small pOrtion of the structural information theoretically accessibleto CXMS. Chemical: cross-linkers targeting the acidic residues Asp/Glu could greatly enhance the power of CXMS. However, it has been difficult to develop chemistries that offer selectivity and efficiency under physiological conditions. Here, we report a class of carboxylate-selective diazo-containing cross-linkers (Diazoker) of which Diazoker 1, with a spacer arm consisting of two ethan1,2-diol units, is the best example. Unlike previously developed carboxylateselective cross-linkers like pimelic acid dihydrazide (PDH), Diazoker 1 does not require a coupling reagent. We tested Diazoker 1 on nine model proteins and found that Diazoker 1 generated an average of 73 cross-linked peptide pairs per protein. Although this is 32% fewer than the number generated by PDH, the Diazoker 1 cross-links have a'higher rate of compatibility with protein crystal structures. From a more complex protein mixture, Diazoker 1 and PDH identified 75 and 76 cross-linked peptide pairs, respectively. The Asp/Glu residues cross-linked by Diazoker 1 are not the same as those cross-linked by PDH. Diazoker 1 favors acidic residues that are less exposed to solvent. In conclusion, Diazoker 1 is complementary to existing cross-linkers and expands the toolkit of CXMS for structural analysis of proteins.
WOS关键词DISTANCE RESTRAINTS ; ACIDIC RESIDUES ; LINKING ; ASSEMBLIES ; BIOCONJUGATION ; BIOINFORMATICS ; COMPLEXES ; REAGENTS ; BIOLOGY ; PROBES
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000423011600024
出版者AMER CHEMICAL SOC
资助机构National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201) ; National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201) ; National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201) ; National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201)
源URL[http://ir.wipm.ac.cn/handle/112942/12786]  
专题中国科学院武汉物理与数学研究所
通讯作者Lei, Xiaoguang; Dong, Meng-Qiu
作者单位1.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Ctr Quantitat Biol, Beijing 100871, Peoples R China
3.NIBS, Beijing 102206, Peoples R China
4.Chinese Acad Med Sci, Beijing 100730, Peoples R China
5.Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China
6.Peking Univ, Beijing Natl Lab Mol Sci,Minist Educ, Key Lab Bioorgan Chem & Mol Engn,Dept Chem Biol, Coll Chem & Mol Engn,Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China
7.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Li, Qiang,Tan, Dan,Wang, Jian-Hua,et al. Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures[J]. ANALYTICAL CHEMISTRY,2018,90(2):1195-1201.
APA Li, Qiang.,Tan, Dan.,Wang, Jian-Hua.,Zhang, Xiaoyun.,Li, Maodong.,...&Gong, Zhou.(2018).Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures.ANALYTICAL CHEMISTRY,90(2),1195-1201.
MLA Li, Qiang,et al."Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures".ANALYTICAL CHEMISTRY 90.2(2018):1195-1201.

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。