中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy

文献类型：期刊论文

作者Ye, Yansheng2; Wu, Qiong2; Zheng, Wenwen2; Jiang, Bin2; Pielak, Gary J.1,3; Liu, Maili2; Li, Conggang2
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2018
卷号410期号:3页码:869-874
ISSN号1618-2642
关键词Proteins Rotational Motion Nmr Viscosity Weak Interactions
DOI10.1007/s00216-017-0745-4
文献子类Article
英文摘要Protein diffusion in living cells might differ significantly from that measured in vitro. Little is known about the effect of globular protein size on rotational diffusion in cells because each protein has distinct surface properties, which result in different interactions with cellular components. To overcome this problem, the B1 domain of protein G (GB1) and several concatemers of the protein were labeled with 5-fluorotryptophan and studied by F-19 NMR in Escherichia coli cells, Xenopus laevis oocytes, and in aqueous solutions crowded with glycerol, or Ficoll70 (TM) and lysozyme. Relaxation data show that the size dependence of protein rotation in cells is due to weak interactions of the target protein with cellular components, but the effect of these interactions decreases as protein size increases. The results provide valuable information for interpreting protein diffusion data acquired in living cells.
WOS关键词MAGNETIC-RESONANCE-SPECTROSCOPY ; XENOPUS-LAEVIS OOCYTES ; LIVING CELLS ; ESCHERICHIA-COLI ; MULTIDIMENSIONAL NMR ; QUINARY STRUCTURE ; IN-VIVO ; DIFFUSION ; CYTOPLASM ; ENVIRONMENTS
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者SPRINGER HEIDELBERG
WOS记录号WOS:000422952600017
资助机构Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854)
源URL[http://ir.wipm.ac.cn/handle/112942/12818]  
专题中国科学院武汉物理与数学研究所
通讯作者Liu, Maili; Li, Conggang
作者单位1.Univ North Carolina Chapel Hill, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China
3.Univ North Carolina Chapel Hill, Dept Chem Biochem & Biophys, Chapel Hill, NC 27599 USA
推荐引用方式
GB/T 7714		 Ye, Yansheng,Wu, Qiong,Zheng, Wenwen,et al. Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(3):869-874.
APA Ye, Yansheng.,Wu, Qiong.,Zheng, Wenwen.,Jiang, Bin.,Pielak, Gary J..,...&Li, Conggang.(2018).Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(3),869-874.
MLA Ye, Yansheng,et al."Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.3(2018):869-874.

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。