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Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain

文献类型：期刊论文

作者Zeng, Hao-Long2; Yu, Fei-Long1; Zhang, Zhijian3; Yang, Qing1; Jin, Sen3; He, Xiaobin3; Chen, Xi4; Shen, Ying2; Cheng, Liming2; Guo, Lin1
刊名BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
出版日期2018-02-01
卷号1866期号:2页码:307-315
关键词Pseudorabies Virus Quantitative Proteomics Nervous System Virus Trafficking Synaptosome
ISSN号1570-9639
DOI10.1016/j.bbapap.2017.11.010
文献子类Article
英文摘要Bartha, the pseudorabies virus (PRV) vaccine strain, is widely used in studies of neuronal circuit-tracing, due to its attenuated virulence and retrograde spreading. However, we know little regarding the molecular mechanisms of PRV infection and spreading between structurally connected neurons. In this study, we systematically analyzed the host brain proteomes after acute infection with PRV, attempting to identified the proteins involved in the processes. Mice were injected with PRV-Bartha and PRV-Becker (PRV-Bartha's wild-type parent strain) in the olfactory system, the proteomes of the brain and synaptosome were analyzed and compared at various infection intervals using mass spectrometry-based proteomics techniques.-In all, we identified > 100 PRV-infection regulated proteins at the whole-tissue level and the synaptosome level. While at whole-tissue level, bioinformatics analyses mapped most of the regulations to the inflammation pathways, at the synaptosome level, most of those to synaptic transmission, cargo transport and cytoskeleton organization. We established regulated protein networks demonstrating distinct cellular regulation pattern between the global and the synaptosome levels. Moreover, we identified a series of potentially PRV-strain-specific regulated proteins with diverse biological functions. This study may provide new clues for molecular mechanisms for PRV infection and spread.
WOS关键词NERVOUS-SYSTEM CIRCUITS ; ALPHA-HERPESVIRUSES ; TRANSPORT ; NEURONS ; PROTEIN ; AXONS ; TRAFFICKING ; PATHWAY ; FUSION ; GOLGI
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000423640800011
出版者ELSEVIER SCIENCE BV
资助机构National Natural Science Foundation of China(91132307/H09) ; National Natural Science Foundation of China(91132307/H09) ; National Basic Research Program (973 Program) of China(2015CB755600) ; National Basic Research Program (973 Program) of China(2015CB755600) ; Natural Science Foundation of China (NSFC)(31600666) ; Natural Science Foundation of China (NSFC)(31600666) ; Strategic Priority Research Program (B)(XDB02050005) ; Strategic Priority Research Program (B)(XDB02050005) ; National Natural Science Foundation of China(91132307/H09) ; National Natural Science Foundation of China(91132307/H09) ; National Basic Research Program (973 Program) of China(2015CB755600) ; National Basic Research Program (973 Program) of China(2015CB755600) ; Natural Science Foundation of China (NSFC)(31600666) ; Natural Science Foundation of China (NSFC)(31600666) ; Strategic Priority Research Program (B)(XDB02050005) ; Strategic Priority Research Program (B)(XDB02050005) ; National Natural Science Foundation of China(91132307/H09) ; National Natural Science Foundation of China(91132307/H09) ; National Basic Research Program (973 Program) of China(2015CB755600) ; National Basic Research Program (973 Program) of China(2015CB755600) ; Natural Science Foundation of China (NSFC)(31600666) ; Natural Science Foundation of China (NSFC)(31600666) ; Strategic Priority Research Program (B)(XDB02050005) ; Strategic Priority Research Program (B)(XDB02050005) ; National Natural Science Foundation of China(91132307/H09) ; National Natural Science Foundation of China(91132307/H09) ; National Basic Research Program (973 Program) of China(2015CB755600) ; National Basic Research Program (973 Program) of China(2015CB755600) ; Natural Science Foundation of China (NSFC)(31600666) ; Natural Science Foundation of China (NSFC)(31600666) ; Strategic Priority Research Program (B)(XDB02050005) ; Strategic Priority Research Program (B)(XDB02050005)
源URL[http://ir.wipm.ac.cn/handle/112942/12832]  
专题中国科学院武汉物理与数学研究所
通讯作者Guo, Lin; Xu, Fuqiang
作者单位1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan, Hubei, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Hosp, Dept Lab Med, Tongji Med Coll, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Ctr Brain Sci,Wuhan Inst Phys & Math,CAS Ctr Exce, Wuhan, Hubei, Peoples R China
4.Wuhan Inst Biotechnol, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Zeng, Hao-Long,Yu, Fei-Long,Zhang, Zhijian,et al. Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2018,1866(2):307-315.
APA Zeng, Hao-Long.,Yu, Fei-Long.,Zhang, Zhijian.,Yang, Qing.,Jin, Sen.,...&Xu, Fuqiang.(2018).Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1866(2),307-315.
MLA Zeng, Hao-Long,et al."Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1866.2(2018):307-315.

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来源：武汉物理与数学研究所

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