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Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats

文献类型：期刊论文

作者Zeng, Hao-Long2; Yang, Qing4; Du, Hongying5; Li, Huijun2; Shen, Ying2; Liu, Taotao2; Chen, Xi4; Kamal, Ghulam Mustafa6; Guan, Qing2; Cheng, Liming2
刊名ONCOTARGET
出版日期2017-11-24
卷号8期号:60页码:102020-102032
关键词Alcohol Preference Liver Mitochondria Quantitative Proteomics Metabolomics Tca Cycle
ISSN号1949-2553
DOI10.18632/oncotarget.22040
文献子类Article
英文摘要Alcohol preference induced tolerance in humans and animals when their bodily functions adapt to compensate for the disruption caused by alcohol consumption. This was thought to be an important component of the genetic predisposition to alcoholism. To investigate the underlying mechanisms of hepatic metabolic tolerance during alcohol preference, the alcohol preferring and alcohol non-preferring rats were used in this study. The liver mitochondria were purified for comparative quantitative proteomics analysis, and the liver metabolite extracts were collected for metabolomics analysis. Our study identified 96 differentially expressed hepatic mitochondrial proteins that associated with alcohol preference, the further gene ontology and protein interaction network analysis suggest a down-regulation of amino acid metabolism and up-regulation of lipid metabolism. We found alcohol preference induced a series of enzymes decreased (e.g. SSADH and GABA-T) and several amino acids increased (e.g. glutamate and aspartate) in rat liver, indicating down-regulations of glutamate degradation occurred during alcohol preference. Most of these changes were due to the genetic differences between alcohol preferring and non-preferring animals. Furthermore, this study would provided new insights to further clarify the mechanisms of hepatic metabolic tolerance during alcohol preference.
WOS关键词LIVER-MITOCHONDRIA ; DRINKING ; DEPENDENCE ; ETHANOL ; EXPOSURE ; BRAIN ; GABA ; PHOSPHORYLATION ; SPECTROSCOPY ; CONSUMPTION
WOS研究方向Oncology ; Cell Biology
语种英语
WOS记录号WOS:000419533200085
出版者IMPACT JOURNALS LLC
资助机构Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193) ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193) ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193) ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193)
源URL[http://ir.wipm.ac.cn/handle/112942/12834]  
专题中国科学院武汉物理与数学研究所
通讯作者Wang, Jie; Xu, Fuqiang
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Hosp, Dept Lab Med, Tongji Med Coll, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance,Key Lab Magnet Resonan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Hubei, Peoples R China
4.Wuhan Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China
5.Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan, Hubei, Peoples R China
6.Univ Faisalabad, Dept Chem, Govt Coll, Faisalabad, Pakistan
7.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Zeng, Hao-Long,Yang, Qing,Du, Hongying,et al. Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats[J]. ONCOTARGET,2017,8(60):102020-102032.
APA Zeng, Hao-Long.,Yang, Qing.,Du, Hongying.,Li, Huijun.,Shen, Ying.,...&Xu, Fuqiang.(2017).Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.ONCOTARGET,8(60),102020-102032.
MLA Zeng, Hao-Long,et al."Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats".ONCOTARGET 8.60(2017):102020-102032.

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来源：武汉物理与数学研究所

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