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Identification, Characteristics and Mechanism of 1-Deoxy-N-acetylglucosamine from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493

文献类型：期刊论文

作者Huang, Dian1; Shao, Zong-Ze4; Yu, Yi3; Cai, Min-Min1; Zheng, Long-Yu1; Li, Guang-Yu4; Yu, Zi-Niu1; Yi, Xian-Feng2; Zhang, Ji-Bin1; Hao, Fu-Hua2
刊名MARINE DRUGS
出版日期2018-02-01
卷号16期号:2
关键词Virgibacillus Dokdonensis Xanthomonas Oryzae Pv Oryzae 1-deoxy-n-acetylglucosamine Antibacterial Characteristics Mechanism
ISSN号1660-3397
DOI10.3390/md16020052
文献子类Article
英文摘要Xanthomonas oryzae pv. oryzae, which causes rice bacterial blight, is one of the most destructive pathogenic bacteria. Biological control against plant pathogens has recently received increasing interest. 1-Deoxy-N-acetylglucosamine (1-DGlcNAc) was extracted from the supernatant of Virgibacillus dokdonensis MCCC 1A00493 fermentation through antibacterial bioassay-guided isolation. Its structure was elucidated by LC/MS, NMR, chemical synthesis and time-dependent density functional theory (TD-DFT) calculations. 1-DGlcNAc specifically suppressed X. oryzae pv. oryzae PXO99A (MIC was 23.90 g/mL), but not other common pathogens including Xanthomonas campestris pv. campestris str.8004 and Xanthomonas oryzae pv. oryzicola RS105. However, its diastereomer (2-acetamido-1,5-anhydro-2-deoxy-d-mannitol) also has no activity to X. oryzae pv. oryzae. This result suggested that activity of 1-DGlcNAc was related to the difference in the spatial conformation of the 2-acetamido moiety, which might be attributed to their different interactions with a receptor. Eighty-four unique proteins were found in X. oryzae pv. oryzae PXO99A compared with the genome of strains8004 and RS105 by blastp. There may be unique interactions between 1-DGlcNAc and one or more of these unique proteins in X. oryzae pv. oryzae. Quantitative real-time PCR and the pharmMapper server indicated that proteins involved in cell division could be the targets in PXO99A. This research suggested that specificity of active substance was based on the active group and spatial conformation selection, and these unique proteins could help to reveal the specific mechanism of action of 1-DGlcNAc against PXO99A.
WOS关键词ORYZAE PV.-ORYZAE ; ANTIBACTERIAL ACTIVITY ; VIRULENCE FACTORS ; GENE-EXPRESSION ; GENOME SEQUENCE ; RICE ; RESISTANCE ; ORYZICOLA ; CHIRALITY ; BACTERIA
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000427528800014
出版者MDPI
资助机构National Basic Research Program of China (973)(2015CB150506) ; National Basic Research Program of China (973)(2015CB150506) ; National Natural Science Foundation(30971884) ; National Natural Science Foundation(30971884) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; National Basic Research Program of China (973)(2015CB150506) ; National Basic Research Program of China (973)(2015CB150506) ; National Natural Science Foundation(30971884) ; National Natural Science Foundation(30971884) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; National Basic Research Program of China (973)(2015CB150506) ; National Basic Research Program of China (973)(2015CB150506) ; National Natural Science Foundation(30971884) ; National Natural Science Foundation(30971884) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; National Basic Research Program of China (973)(2015CB150506) ; National Basic Research Program of China (973)(2015CB150506) ; National Natural Science Foundation(30971884) ; National Natural Science Foundation(30971884) ; Fundamental Research Funds for the Central Universities(2662016PY110) ; Fundamental Research Funds for the Central Universities(2662016PY110)
源URL[http://ir.wipm.ac.cn/handle/112942/12882]  
专题中国科学院武汉物理与数学研究所
通讯作者Zhang, Ji-Bin; Hao, Fu-Hua
作者单位1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Natl Engn Res Ctr Microbe Pesticides, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
3.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430070, Hubei, Peoples R China
4.State Ocean Adm, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Dian,Shao, Zong-Ze,Yu, Yi,et al. Identification, Characteristics and Mechanism of 1-Deoxy-N-acetylglucosamine from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493[J]. MARINE DRUGS,2018,16(2).
APA Huang, Dian.,Shao, Zong-Ze.,Yu, Yi.,Cai, Min-Min.,Zheng, Long-Yu.,...&Hao, Fu-Hua.(2018).Identification, Characteristics and Mechanism of 1-Deoxy-N-acetylglucosamine from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493.MARINE DRUGS,16(2).
MLA Huang, Dian,et al."Identification, Characteristics and Mechanism of 1-Deoxy-N-acetylglucosamine from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493".MARINE DRUGS 16.2(2018).

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来源：武汉物理与数学研究所

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