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Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200

文献类型：期刊论文

作者Zhang, Sheng-Da1,3,4; Santini, Claire-Lise2,3,4; Zhang, Wei-Jia1,3,4; Barbe, Valerie5; Mangenot, Sophie5; Guyomar, Charlotte2,3,4; Garel, Marc6; Chen, Hai-Tao1,3,4; Li, Xue-Gong1,3,4; Yin, Qun-Jian1,3,4
刊名EXTREMOPHILES
出版日期2016-05-01
卷号20期号:3页码:301-310
ISSN号1431-0651
关键词Deep-sea Adaptation Bioluminescence Tmao Reductase Hydrostatic Pressure Anaerobic Respiration
DOI10.1007/s00792-016-0822-1
文献子类Article
英文摘要Bacteria of the genus Photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. Genomic characteristics underlying this variability across species are poorly understood. Here we carried out genomic and physiological analysis of Photobacterium phosphoreum strain ANT-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. Using optical mapping we updated the genomic data and reassembled it into two chromosomes and a large plasmid. Genomic analysis revealed a versatile energy metabolic potential and physiological analysis confirmed its growth capacity by deriving energy from fermentation of glucose or maltose, by respiration with formate as electron donor and trimethlyamine N-oxide (TMAO), nitrate or fumarate as electron acceptors, or by chemo-organo-heterotrophic growth in rich media. Despite that it was isolated at a site with saturated dissolved oxygen, the ANT-2200 strain possesses four gene clusters coding for typical anaerobic enzymes, the TMAO reductases. Elevated hydrostatic pressure enhances the TMAO reductase activity, mainly due to the increase of isoenzyme TorA1. The high copy number of the TMAO reductase isoenzymes and pressure-enhanced activity might imply a strategy developed by bacteria to adapt to deep-sea habitats where the instant TMAO availability may increase with depth.
WOS关键词ESCHERICHIA-COLI ; RESPONSE REGULATOR ; OPERON EXPRESSION ; MARINE BACTERIUM ; PROFUNDUM SS9 ; STRAIN MO-1 ; PRESSURE ; TORR ; ADAPTATION ; SEQUENCE
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
语种英语
出版者SPRINGER JAPAN KK
WOS记录号WOS:000374832100007
资助机构Sanya Institute of Deep-Sea Sciences and Engineering(SIDSSE-201307 ; Strategic Priority Research Program(XDB06010203) ; International Partnership for Innovative Team Program from the Chinese Academy of Sciences(20140491526) ; National Natural Science Foundation of China(NSFC 41506147) ; Centre National de la Recherche Scientifique ; China Ocean Mineral Resources R & D Association (COMRA) Special Foundation(DY125-15-R-03) ; National Science Foundation of China(NSFC 41306161) ; Mt. Tai Scholar Construction Engineering Special Foundation of Shandong Province ; France Genomique ; SIDSSE-QN-201405 ; SIDSSE-QN-201406 ; SIDSSE-QN-201408)
源URL[http://ir.qibebt.ac.cn/handle/337004/10451]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Wu, Long-Fei
作者单位1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Dept Deep Sea Sci, Deep Sea Microbial Cell Biol, Sanya, Peoples R China
2.Aix Marseille Univ, CNRS, IMM, LCB UMR 7257, 31 Chem Joseph Aiguier, F-13402 Marseille 20, France
3.LCB CNRS, France China Biomineralizat & Nanostruct Lab, LIA BioMNSL, Marseille, France
4.Chinese Acad Sci, SIDSSE, Sanya, Peoples R China
5.CEA, DSV IG Genoscope LF, Evry, France
6.Univ Sud Toulon Var, Aix Marseille Univ, CNRS INSU, IRD,MIO,UM110, F-13288 Marseille, France
7.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
8.CEA, DSV, IBEB, SBTN, Bagnols Sur Ceze, France
9.CEA, DSV IG Genoscope, Lab Analyse Bioinformat Genom & Metab, Evry, France
10.CNRS, UMR 8030, Evry, France
推荐引用方式
GB/T 7714		 Zhang, Sheng-Da,Santini, Claire-Lise,Zhang, Wei-Jia,et al. Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200[J]. EXTREMOPHILES,2016,20(3):301-310.
APA Zhang, Sheng-Da.,Santini, Claire-Lise.,Zhang, Wei-Jia.,Barbe, Valerie.,Mangenot, Sophie.,...&Wu, Long-Fei.(2016).Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200.EXTREMOPHILES,20(3),301-310.
MLA Zhang, Sheng-Da,et al."Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200".EXTREMOPHILES 20.3(2016):301-310.

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来源：青岛生物能源与过程研究所

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