A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli
文献类型：期刊论文
| 作者 | Lacmata, Stephen Tamekou1,2; Yao, Lan1; Xian, Mo1; Liu, Hui1; Kuiate, Jules-Roger2; Liu, Huizhou1; Feng, Xinjun1 ; Zhao, Guang1
|
| 刊名 | BIOENGINEERED
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:5页码:594-599 |
| 关键词 | Autolysis System Industrial Applications Magnesium P3hp Promoter Untranslated Regions |
| ISSN号 | 2165-5979 |
| DOI | 10.1080/21655979.2017.1286432 |
| 文献子类 | Article |
| 英文摘要 | The release of intracellular products, especially polyhydroxyalkanoates, is still a great challenge in industry. To solve this bottleneck, a novel autolysis system strictly controlled with magnesium was constructed and applied to poly(3-hydroxypropionate) production in engineered Escherichia coli. The autolysis system was constructed by inserting the 5untranslated region (5UTR) behind promoter PmgtA with lysis genes (S, R, and Rz, from E. coli) overexpressed. The autolysis system functioned well (lysis efficiency of more than 90%) in the P3HP producer with double plasmids containing lysis genes and P3HP biosynthesis genes, whereas the P3HP production was reduced due to plasmid losses. After the autolysis genes and P3HP biosynthesis genes were integrated into one plasmid, the P3HP content of 72.7% (2.4times of the control) and the plasmid stability of 79.8 +/- 3.1% were achieved in strain Q2646 with promoter PmgtA-UTR. However, the strain Q2647 with promoter PmgtA could not accumulate P3HP because of rapid cell lysis. The novel autolysis system activated in Mg2+-depleted conditions proves to be feasible for polyhydroxyalkanoates production, which may have great application potential for other intracellular products. |
| WOS关键词 | POLY(3-HYDROXYPROPIONATE) ; MG2+ ; BIOSYNTHESIS ; GLYCEROL ; RNA |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000413964300017 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | 100-Talent Project of CAS ; Key Program of CAS(ZDRW-ZS-2016-3M ; National Natural Science Foundation of China(31670089 ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; Shandong Province Post-doctoral Innovation Foundation(201603076) ; KGZD-EW-606-1-3) ; 31670493) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10463]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 通讯作者 | Feng, Xinjun |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Dschang, Dept Biochem, Lab Microbiol & Antimicrobials Subst, Fac Sci, Dschang, Cameroon |
| 推荐引用方式 GB/T 7714 | Lacmata, Stephen Tamekou,Yao, Lan,Xian, Mo,et al. A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli[J]. BIOENGINEERED,2017,8(5):594-599. |
| APA | Lacmata, Stephen Tamekou.,Yao, Lan.,Xian, Mo.,Liu, Hui.,Kuiate, Jules-Roger.,...&Zhao, Guang.(2017).A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.BIOENGINEERED,8(5),594-599. |
| MLA | Lacmata, Stephen Tamekou,et al."A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli".BIOENGINEERED 8.5(2017):594-599. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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