Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans
文献类型：期刊论文
| 作者 | Chen, Guoqiang1,2; Wang, Jiming1; Su, Yulong3; Zhu, Youshuang4; Zhang, Ge1,3; Zhao, Hongwei1; Liu, Haobao3; Yang, Ying1; Nian, Rui1; Zhang, Haibo1
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| 刊名 | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 47期号:10页码:963-969 |
| 关键词 | Aureobasidium Pullulans Fermentation Conditions Ftir Nmr Pullulan Tlc |
| ISSN号 | 1082-6068 |
| DOI | 10.1080/10826068.2017.1350979 |
| 文献子类 | Article |
| 英文摘要 | Pullulan with different molecular-weight could be applied in various fields. A UV-induced mutagenesis Aureobasidium pullulans UVMU6-1 was obtained from the strain A. pullulans CGMCC3.933 for the production of low-molecular-weight pullulan. First, the obtained polysaccharide from A. pullulans UVMU6-1 was purified and identified to be pullulan with thin-layer chromatography, Fourier transform infrared, and nuclear magnetic resonance. Then, culture medium and conditions for this strain were optimized by flask fermentation. Based on the optimized medium and culture conditions (pH 4, addition of 4g/L Tween 80 for 96hr of cultivation), continuously fermentation was performed. The highest pullulan production and dry biomass was 109 and 125g/L after fermentation for 114hr, respectively. The average productivity was about 1g/L/hr, which was intensively higher than the previous reported. This study would lay foundations for the industrial production of pullulan. |
| WOS关键词 | MOLECULAR-WEIGHT PULLULAN ; SUGAR METABOLISM ; TWEEN 80 ; FERMENTATION ; STRAIN ; OPTIMIZATION ; POTATO ; LIPASE ; EXOPOLYSACCHARIDES ; POLYSACCHARIDE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000418064000003 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | National Natural Science Foundation of China (NSF)(31400084) ; Natural Science Foundation of Shandong Province(ZR2014CQ008) ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; Youth Innovation Promotion Association (CAS)(2017252) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10521]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 通讯作者 | Zhang, Haibo; Wei, Yuxi; Xian, Mo |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao, Peoples R China 4.Jining Med Univ, Sch Biol Sci, Jining, Peoples R China 5.189 Songling Rd, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Guoqiang,Wang, Jiming,Su, Yulong,et al. Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2017,47(10):963-969. |
| APA | Chen, Guoqiang.,Wang, Jiming.,Su, Yulong.,Zhu, Youshuang.,Zhang, Ge.,...&Xian, Mo.(2017).Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,47(10),963-969. |
| MLA | Chen, Guoqiang,et al."Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 47.10(2017):963-969. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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