Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid
文献类型：期刊论文
| 作者 | Deng, Yunyan1,2; Hu, Zhangxi1,2; Shang, Lixia1; Peng, Quancai3; Tang, Ying Zhong1,2 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2017-12-11 |
| 卷号 | 8 |
| 关键词 | Resting Cyst Harmful Algal Blooms (Habs) Dormancy Uhplc-ms/ms Abscisicacid (Aba) Dinoflagellate Scrippsiella Trochoidea |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2017.02450 |
| 文献子类 | Article |
| 英文摘要 | Due to the vital importance of resting cysts in the biology and ecology of many dinoflagellates, a transcriptomic investigation on Scrippsiella trochoidea was conducted with the aim to reveal the molecular processes and relevant functional genes regulating encystment and dormancy in dinoflagellates. We identified via RNA-seq 3,874 (out of 166,575) differentially expressed genes (DEGs) between resting cysts and vegetative cells; a pause of photosynthesis (confirmed via direct measurement of photosynthetic efficiency); an active catabolism including beta-oxidation, glycolysis, glyoxylate pathway, and TCA in resting cysts (tested via measurements of respiration rate); 12 DEGs encoding meiotic recombination proteins and members of MEI2-like family potentially involved in sexual reproduction and encystment; elevated expressions in genes encoding enzymes responding to pathogens (chitin deacetylase) and ROS stress in cysts; and 134 unigenes specifically expressed in cysts. We paid particular attention to genes pertaining to phytohormone signaling and identified 4 key genes regulating abscisic acid (ABA) biosynthesis and catabolism, with further characterization based on their full-length cDNA obtained via RACE-PCR. The qPCR results demonstrated elevated biosynthesis and repressed catabolism of ABA during the courses of encystment and cyst dormancy, which was significantly enhanced by lower temperature (4 +/- 1 degrees C) and darkness. Direct measurements of ABA using UHPLC-MS/MS and ELISA in vegetative cells and cysts both fully supported qPCR results. These results collectively suggest a vital role of ABA in regulating encystment and maintenance of dormancy, akin to its function in seed dormancy of higher plants. Our results provided a critical advancement in understanding molecular processes in resting cysts of dinoflagellates. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; ALGA HAEMATOCOCCUS-PLUVIALIS ; SEED DORMANCY ; GENE-EXPRESSION ; RNA-SEQ ; ZEAXANTHIN EPOXIDASE ; ARABIDOPSIS-THALIANA ; AKASHIWO-SANGUINEA ; CYST PRODUCTION ; KARENIA-BREVIS |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000417577500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(U1606404) ; National Science Foundation of China(41476142 ; Scientific and Technological Innovation Project ; Qingdao National Laboratory for Marine Science and Technology(2016ASKJ02) ; 41606126 ; 61533011 ; 41506143 ; U1301235) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10523]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Tang, Ying Zhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Res Ctr Anal & Measurement, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,et al. Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid[J]. FRONTIERS IN MICROBIOLOGY,2017,8. |
| APA | Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,Peng, Quancai,&Tang, Ying Zhong.(2017).Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid.FRONTIERS IN MICROBIOLOGY,8. |
| MLA | Deng, Yunyan,et al."Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid".FRONTIERS IN MICROBIOLOGY 8(2017). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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