Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps
文献类型：期刊论文
| 作者 | Wen, Li-lian1,2,3; Zhao, Ming-li1,2,3; Chi, Heng1,2; Sun, Li1,2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 72页码:470-476 |
| 关键词 | Neutrophil Extracellular Traps Histone Elastase Cynoglossus Semilaevis Antimicrobial |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2017.11.004 |
| 文献子类 | Article |
| 英文摘要 | Neutrophil extracellular traps (NETs) are a form of extracellular antimicrobial structure of neutrophils observed in higher and lower vertebrates, the latter including the teleost fish tongue sole Cynoglossus semilaevis. However, the antimicrobial mechanism of fish NETs is unknown. In the present study, we examined the potential contribution of histones and elastases to the antibacterial effect of tongue sole NETs. For this purpose, two histones (CsH2B and CsH4) and two elastases (CsElal and CsEla2) of tongue sole were investigated. The histones and elastases possess the conserved domain structures characteristic of that of histones H2B/H4 and trypsin-like serine protease, respectively. Recombinant CsH2B, CsH4, CsElal, and CsEla2 bound a wide range of Gram-negative and Gram-positive bacteria, and some of the bound bacteria were inhibited in growth by the bound histones/elastases. CsH2B, CsH4, CsElal, and CsEla2 were all localized in NETs induced by various stimuli including bacterial pathogen. Treatment of NETs with antibodies targeting CsH2B, CsH4, CsElal, and CsEla2 significantly reduced the antimicrobial effect of NETs. These results indicate that histones and chymotrypsin-like elastases are fundamental components of teleost NETs that play important roles in the antimicrobial activity of NETs. |
| WOS关键词 | CYNOGLOSSUS-SEMILAEVIS ; BACTERIAL-INFECTION ; SCOPHTHALMUS-MAXIMUS ; HUMAN-GRANULOCYTES ; HOST-DEFENSE ; PROTEINS ; LEUKOCYTE ; GRANULES ; KIDNEY ; DEGRADATION |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000423644600055 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China(41576150 ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Taishan Scholar Program of Shandong Province ; 31330081) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10565]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Li-lian,Zhao, Ming-li,Chi, Heng,et al. Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:470-476. |
| APA | Wen, Li-lian,Zhao, Ming-li,Chi, Heng,&Sun, Li.(2018).Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps.FISH & SHELLFISH IMMUNOLOGY,72,470-476. |
| MLA | Wen, Li-lian,et al."Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps".FISH & SHELLFISH IMMUNOLOGY 72(2018):470-476. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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