Rational design of engineered microbial cell surface multi-enzyme co-display system for sustainable NADH regeneration from low-cost biomass
文献类型：期刊论文
| 作者 | Han, Lei1 ; Liang, Bo2,3; Song, Jianxia2
|
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 45期号:2页码:111-121 |
| 关键词 | Microbial Cell Surface Display Technology Multi-enzyme Co-display System Cofactor Regeneration System Low-cost Biomass Lactic Acid |
| ISSN号 | 1367-5435 |
| DOI | 10.1007/s10295-018-2002-z |
| 文献子类 | Article |
| 英文摘要 | As an important cofactor, NADH is essential for most redox reactions and biofuel cells. However, supply of exogenous NADH is challenged, due to the low production efficiency and high cost of NADH regeneration system, as well as low stability of NADH. Here, we constructed a novel cell surface multi-enzyme co-display system with ratio- and space-controllable manner as exogenous NADH regeneration system for the sustainable NADH production from low-cost biomass. Dockerin-fused glucoamylase (GA) and glucose dehydrogenase (GDH) were expressed and assembled on the engineered bacterial surfaces, which displayed protein scaffolds with various combinations of different cohesins. When the ratio of GA and GDH was 3:1, the NADH production rate of the whole-cell biocatalyst reached the highest level using starch as substrate, which was three times higher than that of mixture of free enzymes, indicating that the highly ordered spatial organization of enzymes would promote reactions, due to the ratio of enzymes and proximity effect. To confirm performance of the established NADH regeneration system, the highly efficient synthesis of l-lactic acid (l-LA) was conducted by the system and the yield of l-LA (16 g/L) was twice higher than that of the mixture of free enzymes. The multi-enzyme co-display system showed good stability in the cyclic utilization. In conclusion, the novel sustainable NADH system would provide a cost-effective strategy to regenerate cofactor from low-cost biomass. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; ETHANOL-PRODUCTION ; CLOSTRIDIUM-THERMOCELLUM ; COFACTOR REGENERATION ; CELLULOSIC ETHANOL ; LACTIC-ACID ; STRATEGIES ; CONVERSION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000423696900004 |
| 出版者 | SPRINGER HEIDELBERG |
| 资助机构 | National Natural Science Foundation of China(21705087) ; Distinguished Scholars of Qingdao Agricultural University(6631117015) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10579]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 通讯作者 | Han, Lei; Liang, Bo |
| 作者单位 | 1.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, 700 Changcheng Rd, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, 189 Songling Rd, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Lei,Liang, Bo,Song, Jianxia. Rational design of engineered microbial cell surface multi-enzyme co-display system for sustainable NADH regeneration from low-cost biomass[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2018,45(2):111-121. |
| APA | Han, Lei,Liang, Bo,&Song, Jianxia.(2018).Rational design of engineered microbial cell surface multi-enzyme co-display system for sustainable NADH regeneration from low-cost biomass.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,45(2),111-121. |
| MLA | Han, Lei,et al."Rational design of engineered microbial cell surface multi-enzyme co-display system for sustainable NADH regeneration from low-cost biomass".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 45.2(2018):111-121. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。