Function analysis of 5 '-UTR of the cellulosomal xyl-doc cluster in Clostridium papyrosolvens
文献类型：期刊论文
| 作者 | Zou, Xia1; Ren, Zhenxing2; Wang, Na3; Cheng, Yin3; Jiang, Yuanyuan3; Wang, Yan1; Xu, Chenggang3,4,5
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| 刊名 | BIOTECHNOLOGY FOR BIOFUELS
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| 出版日期 | 2018-02-16 |
| 卷号 | 11 |
| ISSN号 | 1754-6834 |
| 关键词 | Clostridium Papyrosolvens Electrotransformation Xyl-doc Gene Cluster Promoter 5 ' Untranslated Region (5 '-utr) |
| DOI | 10.1186/s13068-018-1040-0 |
| 文献子类 | Article |
| 英文摘要 | Background: Anaerobic, mesophilic, and cellulolytic Clostridium papyrosolvens produces an efficient cellulolytic extracellular complex named cellulosome that hydrolyzes plant cell wall polysaccharides into simple sugars. Its genome harbors two long cellulosomal clusters: cip-cel operon encoding major cellulosome components (including scaffolding) and xyl-doc gene cluster encoding hemicellulases. Compared with works on cip-cel operon, there are much fewer studies on xyl-doc mainly due to its rare location in cellulolytic clostridia. Sequence analysis of xyl-doc revealed that it harbors a 5' untranslated region (5'-UTR) which potentially plays a role in the regulation of downstream gene expression. Here, we analyzed the function of 5'-UTR of xyl-doc cluster in C. papyrosolvens in vivo via transformation technology developed in this study. |
| WOS关键词 | NATURAL GENETIC-TRANSFORMATION ; ACETOBUTYLICUM ATCC 824 ; REBASE-A DATABASE ; STREPTOCOCCUS-MUTANS ; MODIFICATION ENZYMES ; DNA RESTRICTION ; CELLULOLYTICUM ; COMPETENCE ; PROTEINS ; BIOFILMS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| 出版者 | BIOMED CENTRAL LTD |
| WOS记录号 | WOS:000425983900001 |
| 资助机构 | National Natural Science Foundation of China(31571282 ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2016192) ; Major Program of Shandong Province in China(2015ZDJS04002) ; Fundamental Research Funds for the Central Universities of China(21615458) ; 41676144 ; 41276155) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/10625]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Wang, Yan; Xu, Chenggang |
| 作者单位 | 1.Jinan Univ, Coll Life Sci & Technol, Res Ctr Harmful Algae & Marine Biol, Guangzhou 510632, Guangdong, Peoples R China 2.Shanxi Univ, Inst Appl Chem, Taiyuan 030006, Shanxi, Peoples R China 3.Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Shanxi, Peoples R China 4.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 5.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Xia,Ren, Zhenxing,Wang, Na,et al. Function analysis of 5 '-UTR of the cellulosomal xyl-doc cluster in Clostridium papyrosolvens[J]. BIOTECHNOLOGY FOR BIOFUELS,2018,11. |
| APA | Zou, Xia.,Ren, Zhenxing.,Wang, Na.,Cheng, Yin.,Jiang, Yuanyuan.,...&Xu, Chenggang.(2018).Function analysis of 5 '-UTR of the cellulosomal xyl-doc cluster in Clostridium papyrosolvens.BIOTECHNOLOGY FOR BIOFUELS,11. |
| MLA | Zou, Xia,et al."Function analysis of 5 '-UTR of the cellulosomal xyl-doc cluster in Clostridium papyrosolvens".BIOTECHNOLOGY FOR BIOFUELS 11(2018). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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